Amplification and sequencing of Trichuris trichiura ancient DNA extracted from archaeological sediments

被引：37
作者：
Oh, Chang Seok
Seo, Min [2 ]
Chai, Jong Yil [4 ]
Lee, Sang Jun
Kim, Myeung Ju [3 ]
Park, Jun Bum [5 ]
Shin, Dong Hoon [1 ]
机构：
[1] Seoul Natl Univ, Coll Med, Lab Paleopathol, Inst Forens Med,Dept Anat, Seoul 110799, South Korea
[2] Dankook Univ, Coll Med, Dept Parasitol, Cheonan 330714, South Korea
[3] Dankook Univ, Coll Med, Dept Anat, Cheonan 330714, South Korea
[4] Seoul Natl Univ, Coll Med, Dept Parasitol, Seoul 110799, South Korea
[5] Hangang Inst Cultural Heritage, Seoul 110799, South Korea
来源：
JOURNAL OF ARCHAEOLOGICAL SCIENCE | 2010年 / 37卷 / 06期
关键词：
Trichuris trichiura; Ancient DNA; Korea; PCR; Cloning and sequencing; ENTEROBIUS-VERMICULARIS; HUMAN COPROLITES; EGGS; ASCARIS;
D O I：
10.1016/j.jas.2009.12.029
中图分类号：
Q98 [人类学];
学科分类号：
030303 ;
摘要：
Despite high prevalence of Trichuris trichiura infection, PCR-based analysis on T trichiura from archaeological samples has not been established so far. In the present study, we sought to perform PCR-based amplification of T. trichiura aDNA using the sediments from medieval tomb of Korea. The presence of Trichuris eggs were first detected by microscopic observation; then confirmed by PCR-based aDNA analysis. Obtained sequence showed 100% homology to that of the small subunit ribosomal RNA (SSUrRNA) gene of T. trichiura but distinct from that of other Trichuris species. PCR-based aDNA analysis in this study can serve as effective method to confirm the presence of T trichiura eggs in the soils or coprolites collected from archaeological sites. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1269 / 1273
页数：5
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