Effects of dietary chitosan on growth, lipid metabolism, immune response and antioxidant-related gene expression in Misgurnus anguillicaudatus

被引:68
|
作者
Yan, J. [1 ]
Guo, C. [1 ]
Dawood, M. A. O. [2 ]
Gao, J. [1 ]
机构
[1] Huazhong Agr Univ, Key Lab Agr Anim Genet Breeding & Reprod, Key Lab Freshwater Anim Breeding, Coll Fisheries,Minist Educ,Minist Agr, Wuhan 430070, Peoples R China
[2] Kafrelsheikh Univ, Fac Aquat & Fisheries Sci, El Gish St, Qism Kafr El Shaikh, Kafr El Shaikh, Egypt
基金
中国国家自然科学基金;
关键词
chitosan; Misgurnus anguillicaudatus; gut microbiota; antioxidant ability; lipid metabolism; RED-SEA BREAM; KILLED LACTOBACILLUS-PLANTARUM; FATTY-ACID; DISEASE RESISTANCE; NONSPECIFIC IMMUNITY; CYPRINUS-CARPIO; INNATE IMMUNITY; PAGRUS-MAJOR; PERFORMANCE; SUPPLEMENTATION;
D O I
10.3920/BM2016.0177
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
This study was performed to evaluate the effects of dietary chitosan supplementation on growth performance, lipid metabolism, gut microbial, antioxidant status and immune responses of juvenile loach (Misgurnus anguillicaudatus). Five experimental diets were formulated to contain graded levels of chitosan (0 (control), 0.5, 1, 2 and 5% CHI) for 50 days. Results of the present study showed that body weight gain was significantly higher in fish fed chitosan supplemented diets in dose dependent manner than control group. Increasing dietary chitosan levels reduced gut lipid content. Meanwhile the mRNA expression levels of intestine lipoprotein lipase and fatty acid binding protein 2 were significantly reduced with incremental dietary chitosan level. The percentages of total monounsaturated fatty acid decreased, while polyunsaturated fatty acid increased with dietary chitosan. The fish fed 0.5% CHI had higher mucus lysozyme activity (LZM) than those fed 0% CHI, but the LZM activity was significantly decreased with advancing chitosan supplement. The expression levels of superoxide dismutase, catalase and glutathione peroxidase revealed a similar trend, where the highest expressions were found in fish fed 5% CHI diet. In the term of intestine microbiota between 0 and 1% CHI groups, the proportion of bacteria in the phylum Bacteroidetes increased, whereas the proportion of bacteria in the phylum Firmicutes decreased as the fish supplemented chitosan. In conclusion, supplementation of chitosan improved growth performance, antioxidant status and immunological responses in loach.
引用
收藏
页码:439 / 449
页数:11
相关论文
共 50 条
  • [41] Impact of dietary protein on lipid metabolism-related gene expression in porcine adipose tissue
    Sumei Zhao
    Jing Wang
    Xinlei Song
    Xi Zhang
    Changrong Ge
    Shizheng Gao
    Nutrition & Metabolism, 7
  • [42] Impact of dietary protein on lipid metabolism-related gene expression in porcine adipose tissue
    Zhao, Sumei
    Wang, Jing
    Song, Xinlei
    Zhang, Xi
    Ge, Changrong
    Gao, Shizheng
    NUTRITION & METABOLISM, 2010, 7
  • [43] An Investigation on the Effects of Dietary Vitamin E on Juvenile Sea Urchin (Strongylocentrotus intermedius): Growth, Intestinal Microbiota, Immune Response, and Related Gene Expression
    Li, Min
    Gou, Dan
    Gong, Panke
    Di, Weixiao
    Wang, Lina
    Ding, Jun
    Chang, Yaqing
    Zuo, Rantao
    BIOLOGY-BASEL, 2023, 12 (12):
  • [44] Effects of different dietary lipid sources (perilla, fish, and soybean oils) on growth, lipid metabolism, antioxidant, and immune status in Chinese giant salamander (Andrias davidianus)
    Guo, Yi-Hua
    Ma, Jun-Kang
    Xu, Hang-zhong
    Li, Wei-long
    Liu, Chang-Jiang
    Lu, Hua
    Li, Hong
    Zhai, Xu-liang
    Xue, Yang
    Luo, Li
    FRONTIERS IN MARINE SCIENCE, 2023, 10
  • [45] Effects of dietary tea polyphenols on growth, biochemical and antioxidant responses, fatty acid composition and expression of lipid metabolism related genes of large yellow croaker (Larimichthys crocea)
    Ji, Renlei
    Li, Yicong
    Li, Xueshan
    Xiang, Xiaojun
    Li, Yongnan
    Zhu, Si
    Yang, Bo
    Zhang, Yanjiao
    Mai, Kangsen
    Ai, Qinghui
    AQUACULTURE RESEARCH, 2018, 49 (03) : 1210 - 1218
  • [46] Effects of dietary lipid sources on growth performance, lipid metabolism and antioxidant status of juvenile Russian sturgeon Acipenser gueldenstaedtii
    Li, Q.
    Zhu, H. Y.
    Wei, J. J.
    Zhang, F.
    Li, E. C.
    Du, Z. Y.
    Qin, J. G.
    Chen, L. Q.
    AQUACULTURE NUTRITION, 2017, 23 (03) : 500 - 510
  • [47] Effects of dietary lipid levels on growth, fatty acid composition, antioxidant status and lipid metabolism in juvenile Onychostoma macrolepis
    Gou, Nina
    Chang, Zhiguang
    Deng, Wei
    Ji, Hong
    Zhou, Jishu
    AQUACULTURE RESEARCH, 2019, 50 (11) : 3369 - 3381
  • [48] Effects of dietary polyphenols on mucosal and humoral immune responses, antioxidant defense and growth gene expression in beluga sturgeon (Huso huso)
    Safari, Roghieh
    Hoseinifar, Seyed Hossein
    Imanpour, Mohammad Reza
    Mazandarani, Mohammad
    Sanchouli, Habibollah
    Paolucci, Marina
    AQUACULTURE, 2020, 528
  • [49] Glutamine metabolism and its effects on immune response: molecular mechanism and gene expression
    de Oliveira D.C.
    da Silva Lima F.
    Sartori T.
    Santos A.C.A.
    Rogero M.M.
    Fock R.A.
    Nutrire, 41 (1)
  • [50] Effects of dietary lysine levels on growth performance, whole body composition and gene expression related to glycometabolism and lipid metabolism in grass carp, Ctenopharyngodon idellus fry
    Huang, Dongyu
    Liang, Hualiang
    Ren, Mingchun
    Ge, Xianping
    Ji, Ke
    Yu, Heng
    Maulu, Sahya
    AQUACULTURE, 2021, 530