Effects of dietary chitosan on growth, lipid metabolism, immune response and antioxidant-related gene expression in Misgurnus anguillicaudatus

被引:68
|
作者
Yan, J. [1 ]
Guo, C. [1 ]
Dawood, M. A. O. [2 ]
Gao, J. [1 ]
机构
[1] Huazhong Agr Univ, Key Lab Agr Anim Genet Breeding & Reprod, Key Lab Freshwater Anim Breeding, Coll Fisheries,Minist Educ,Minist Agr, Wuhan 430070, Peoples R China
[2] Kafrelsheikh Univ, Fac Aquat & Fisheries Sci, El Gish St, Qism Kafr El Shaikh, Kafr El Shaikh, Egypt
基金
中国国家自然科学基金;
关键词
chitosan; Misgurnus anguillicaudatus; gut microbiota; antioxidant ability; lipid metabolism; RED-SEA BREAM; KILLED LACTOBACILLUS-PLANTARUM; FATTY-ACID; DISEASE RESISTANCE; NONSPECIFIC IMMUNITY; CYPRINUS-CARPIO; INNATE IMMUNITY; PAGRUS-MAJOR; PERFORMANCE; SUPPLEMENTATION;
D O I
10.3920/BM2016.0177
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
This study was performed to evaluate the effects of dietary chitosan supplementation on growth performance, lipid metabolism, gut microbial, antioxidant status and immune responses of juvenile loach (Misgurnus anguillicaudatus). Five experimental diets were formulated to contain graded levels of chitosan (0 (control), 0.5, 1, 2 and 5% CHI) for 50 days. Results of the present study showed that body weight gain was significantly higher in fish fed chitosan supplemented diets in dose dependent manner than control group. Increasing dietary chitosan levels reduced gut lipid content. Meanwhile the mRNA expression levels of intestine lipoprotein lipase and fatty acid binding protein 2 were significantly reduced with incremental dietary chitosan level. The percentages of total monounsaturated fatty acid decreased, while polyunsaturated fatty acid increased with dietary chitosan. The fish fed 0.5% CHI had higher mucus lysozyme activity (LZM) than those fed 0% CHI, but the LZM activity was significantly decreased with advancing chitosan supplement. The expression levels of superoxide dismutase, catalase and glutathione peroxidase revealed a similar trend, where the highest expressions were found in fish fed 5% CHI diet. In the term of intestine microbiota between 0 and 1% CHI groups, the proportion of bacteria in the phylum Bacteroidetes increased, whereas the proportion of bacteria in the phylum Firmicutes decreased as the fish supplemented chitosan. In conclusion, supplementation of chitosan improved growth performance, antioxidant status and immunological responses in loach.
引用
收藏
页码:439 / 449
页数:11
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