Effects of dietary chitosan on growth, lipid metabolism, immune response and antioxidant-related gene expression in Misgurnus anguillicaudatus

被引:68
|
作者
Yan, J. [1 ]
Guo, C. [1 ]
Dawood, M. A. O. [2 ]
Gao, J. [1 ]
机构
[1] Huazhong Agr Univ, Key Lab Agr Anim Genet Breeding & Reprod, Key Lab Freshwater Anim Breeding, Coll Fisheries,Minist Educ,Minist Agr, Wuhan 430070, Peoples R China
[2] Kafrelsheikh Univ, Fac Aquat & Fisheries Sci, El Gish St, Qism Kafr El Shaikh, Kafr El Shaikh, Egypt
基金
中国国家自然科学基金;
关键词
chitosan; Misgurnus anguillicaudatus; gut microbiota; antioxidant ability; lipid metabolism; RED-SEA BREAM; KILLED LACTOBACILLUS-PLANTARUM; FATTY-ACID; DISEASE RESISTANCE; NONSPECIFIC IMMUNITY; CYPRINUS-CARPIO; INNATE IMMUNITY; PAGRUS-MAJOR; PERFORMANCE; SUPPLEMENTATION;
D O I
10.3920/BM2016.0177
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
This study was performed to evaluate the effects of dietary chitosan supplementation on growth performance, lipid metabolism, gut microbial, antioxidant status and immune responses of juvenile loach (Misgurnus anguillicaudatus). Five experimental diets were formulated to contain graded levels of chitosan (0 (control), 0.5, 1, 2 and 5% CHI) for 50 days. Results of the present study showed that body weight gain was significantly higher in fish fed chitosan supplemented diets in dose dependent manner than control group. Increasing dietary chitosan levels reduced gut lipid content. Meanwhile the mRNA expression levels of intestine lipoprotein lipase and fatty acid binding protein 2 were significantly reduced with incremental dietary chitosan level. The percentages of total monounsaturated fatty acid decreased, while polyunsaturated fatty acid increased with dietary chitosan. The fish fed 0.5% CHI had higher mucus lysozyme activity (LZM) than those fed 0% CHI, but the LZM activity was significantly decreased with advancing chitosan supplement. The expression levels of superoxide dismutase, catalase and glutathione peroxidase revealed a similar trend, where the highest expressions were found in fish fed 5% CHI diet. In the term of intestine microbiota between 0 and 1% CHI groups, the proportion of bacteria in the phylum Bacteroidetes increased, whereas the proportion of bacteria in the phylum Firmicutes decreased as the fish supplemented chitosan. In conclusion, supplementation of chitosan improved growth performance, antioxidant status and immunological responses in loach.
引用
收藏
页码:439 / 449
页数:11
相关论文
共 50 条
  • [1] Effects of dietary protein and lipid levels on growth performance, fatty acid composition and antioxidant-related gene expressions in juvenile loach Misgurnus anguillicaudatus
    Yan, Jie
    Li, Yang
    Liang, Xiao
    Zhang, Yin
    Dawood, Mahmoud A. O.
    Matuli'c, Daniel
    Gao, Jian
    AQUACULTURE RESEARCH, 2017, 48 (10) : 5385 - 5393
  • [2] Effects of dietary highly unsaturated fatty acid levels on growth, fatty acid profiles, antioxidant activities, mucus immune responses and hepatic lipid metabolism related gene expressions in loach (Misgurnus anguillicaudatus) juveniles
    Zhao, Yan
    Wang, Lingkai
    Gao, Jian
    AQUACULTURE RESEARCH, 2019, 50 (09) : 2486 - 2495
  • [3] Effects of dietary of bile acid on growth performance, antioxidant capacity, liver and intestinal health and immune function in loach ( Misgurnus anguillicaudatus)
    Liu, Haihui
    Zhang, Xinyu
    Li, Ke
    Fang, Qiongya
    Liu, Xiaorui
    Sun, Li
    Guo, Guiliang
    An, Siyu
    Li, Min
    Wang, Guiqin
    Kong, Yidi
    AQUACULTURE REPORTS, 2024, 39
  • [4] Effects of dietary quercetin on growth, antioxidant capacity, immune response and immune-related gene expression in snakehead fish, Channa argus
    Kong, Yuxin
    Tian, Jiaxin
    Niu, Xiaotian
    Li, Min
    Kong, Yidi
    Li, Ruoming
    Chen, Xiumei
    Wang, Guiqin
    AQUACULTURE REPORTS, 2022, 26
  • [5] Effects of different dietary vitamin C supplementations on growth performance, mucus immune responses and antioxidant status of loach (Misgurnus anguillicaudatus Cantor) juveniles
    Zhao, Yan
    Zhao, Jiaxing
    Zhang, Yin
    Gao, Jian
    AQUACULTURE RESEARCH, 2017, 48 (08) : 4112 - 4123
  • [6] Growth Response and Gene Expression in Antioxidant-related Enzymes in Two Bermudagrass Genotypes Differing in Salt Tolerance
    Hu, Longxing
    Huang, Zehui
    Liu, Shuilian
    Fu, Jinmin
    JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE, 2012, 137 (03) : 134 - 143
  • [7] Growth performance, fatty-acid composition, lipid deposition and hepatic-lipid metabolism-related gene expression in juvenile pond loach Misgurnus anguillicaudatus fed diets with different dietary soybean oil levels
    Li, Y.
    Jia, Z.
    Liang, X.
    Matulic, D.
    Hussein, M.
    Gao, J.
    JOURNAL OF FISH BIOLOGY, 2018, 92 (01) : 17 - 33
  • [8] Effects of dietary methionine on productivity, reproductive performance, antioxidant capacity, ovalbumin and antioxidant-related gene expression in laying duck breeders
    Ruan, Dong
    Fouad, Ahmed Mohamed
    Fan, Qiuli
    Xia, Weiguang
    Wang, Shuang
    Chen, Wei
    Lin, Chuxiao
    Wang, Ying
    Yang, Lin
    Zheng, Chuntian
    BRITISH JOURNAL OF NUTRITION, 2018, 119 (02) : 121 - 130
  • [9] Effects of dietary phospholipid levels on growth performance, fatty acid composition and antioxidant responses of Dojo loach Misgurnus anguillicaudatus larvae
    Gao, Jian
    Koshio, Shunsuke
    Wang, Weimin
    Li, Yang
    Huang, Songqian
    Cao, Xiaojuan
    AQUACULTURE, 2014, 426 : 304 - 309
  • [10] Effect of Dietary Sugarcane Bagasse Supplementation on Growth Performance, Immune Response, and Immune and Antioxidant-Related Gene Expressions of Nile Tilapia (Oreochromis niloticus) Cultured under Biofloc System
    Lumsangkul, Chompunut
    Tapingkae, Wanaporn
    Sringarm, Korawan
    Jaturasitha, Sanchai
    Xuan, Chinh Le
    Wannavijit, Supreya
    Outama, Piyatida
    Van Doan, Hien
    ANIMALS, 2021, 11 (07):