Metallothionein mediates gene expression of 3.1 mRNA (PTZ17) related to epileptic seizure

被引:14
作者
Miura, N [1 ]
Naganuma, A [1 ]
机构
[1] Tohoku Univ, Grad Sch Pharmaceut Sci, Lab Mol & Biochem Toxicol, Sendai, Miyagi 9808578, Japan
关键词
metallothionein; gene expression; NM31; 3.1; mRNA; PTZ; 17; stellate cell;
D O I
10.1016/S0014-5793(00)01899-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Genes differentially expressed in association with disruption of the metallothionein gene were screened using two hepatic stellate cell lines isolated and established from the livers of normal 129/Sv (IMS/N cells) and transgenic mice deficient in the genes for metallothionein-I and -II (IMS/MT (-) cells), We found one cDNA (tentatively named NM31) that was expressed only in IMS/N cells. Transfecting IMS/MT (-) cells with the genes for both metallothionein-I and -II resulted in NM31 expression. These results suggest that metallothionein is essential for NM31 gene expression. The nucleotide sequence of NM31 (294 bp) was identical to the 3' region of 3.1 mRNA (PTZ 17), which is abundant in the embryonic mouse brain and is related to chemically induced seizures. The present study indicates that metallothionein mediates the expression of specific genes. This is a novel explanation for some of the functions of metallothionein. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:146 / 148
页数:3
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