Discrimination between Theileria lestoquardi and Theileria annulata in their vectors and hosts by RFLP based on the 18S rRNA gene

被引：9
作者：
Spitalska, E
Torina, A
Cannella, V
Caracappa, S
Sparagano, OAE [1 ]
机构：
[1] Univ Newcastle Upon Tyne, Sch Agr Food & Rural Dev, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England
[2] Slovak Acad Sci, Bratislava, Slovakia
[3] Ist Zooprofilatt Sperimentale Sicilia, I-90124 Palermo, Italy
来源：
PARASITOLOGY RESEARCH | 2004年 / 94卷 / 04期
关键词：
D O I：
10.1007/s00436-004-1217-2
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Theileria lestoquardi and T. annulata can occur in similar vectors, and current available probes based on the 18S rRNA gene showed cross-reaction between the two species. However, we developed a species-specific RFLP test based on the MspI restriction enzyme, able to cut amplified products from T. lestoquardi only and to discriminate the two species in both tick and blood samples.
引用
收藏
页码：318 / 320
页数：3
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