Gene expression microarrays and respiratory muscles

被引:6
作者
van Lunteren, Erik
Leahy, Patrick
机构
[1] Cleveland VA Med Ctr, Div Pulm Crit Care & Sleep Med, Cleveland, OH 44106 USA
[2] Case Western Reserve Univ, Cleveland, OH 44106 USA
[3] Case Western Reserve Univ, Sch Med, Case Comprehens Canc Ctr, Gene Express & Genotyping Core Facil, Cleveland, OH 44106 USA
关键词
diaphragm; muscular dystrophy; skeletal muscle; mechanical ventilation; RNA; transcription; MUSCULAR-DYSTROPHY; SKELETAL-MUSCLE; MOUSE MODEL; MDX; PATTERNS; MYOPATHY; SAMPLES;
D O I
10.1016/j.resp.2006.11.007
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
The routine measurement of the expression of tens of thousands of gene transcripts, simultaneously, is a defining advance of the last decade which has been made possible by microarray technology. Using this very powerful approach, a pattern has emerged from a number of studies that suggest a molecular niche for the diaphragm which is quite different from that occupied by limb muscle. All indications are that this is true not only in regard to differential gene transcription patterns in healthy muscles but also in the changes in transcription occurring in association with different diseases. Furthermore, respiratory muscle mounts a rich gene expression response to a number of disturbances, be they primary genetic defects (e.g. various types of muscular dystrophies) or non-genetic perturbations (e.g. controlled mechanical ventilation). Large numbers of genes undergo altered levels of transcription, ranging from tens to hundreds (typical) to thousands. These genes are involved in diverse cellular processes, such as contraction, intermediate metabolism, oxidative stress, apoptosis and cellular adhesion. Functional groups of genes identified as having changed expression differ in many respects from one disease to another. Previously identified pathways of muscle injury and repair are often perturbed to greater extents than previously anticipated, and processes not previously suspected of having important roles in the pathophysiology of specific disorders have been identified. Elucidation of these under-appreciated molecular events may lead to novel therapeutic interventions based on disrupting the downstream adverse consequences of the primary event or facilitating events which ameliorate the injury and/or promote muscle healing. (c) 2006 Elsevier B.V All rights reserved.
引用
收藏
页码:103 / 115
页数:13
相关论文
共 41 条
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PHYSIOLOGICAL GENOMICS, 2006, 25 (01) :85-95