Extraordinary tunable dynamic range of electrochemical aptasensor for accurate detection of ochratoxin A in food samples

被引：11

作者：

Cheng, Lin ^{[1
]}

Qu, Hao ^{[1
]}

Teng, Jun ^{[1
,2
]}

Yao, Li ^{[1
]}

Xue, Feng ^{[2
]}

Chen, Wei ^{[1
]}

机构：

[1] Hefei Univ Technol, Sch Food Sci & Engn, Hefei 230009, Anhui, Peoples R China

[2] Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Jiangsu, Peoples R China

来源：

FOOD SCIENCE AND HUMAN WELLNESS | 2017年 / 6卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Electrochemical aptasensor; Tunable dynamic detection range; OTA detection; Extraordinary dynamic range;

D O I：

10.1016/j.fshw.2017.04.002

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

We report the design of a sensitive, electrochemical aptasensor for detection of ochratoxin A (OTA) with an extraordinary tunable dynamic sensing range. This electrochemical aptasensor is constructed based on the target induced aptamer-folding detection mechanism and the recognition between OTA and its aptamers results in the conformational change of the aptamer probe and thus signal changes for measurement. The dynamic sensing range of the electrochemical aptasensor is successfully tuned by introduction of free assistant aptamer probes in the sensing system. Our electrochemical aptasensor shows an extraordinary dynamic sensing range of 11-order magnitude of OTA concentration from 10(-8) to 10(2) ng/g. Of great significance, the signal response in all OTA concentration ranges is at the same current scale, demonstrating that our sensing protocol in this research could be applied for accurate detections of OTA in a broad range without using any complicated treatment of signal amplification. Finally, OTA spiked red wine and maize samples in different dynamic sensing ranges are determined with the electrochemical aptasensor under optimized sensing conditions. This tuning strategy of dynamic sensing range may offer a promising platform for electrochemical aptasensor optimizations in practical applications. (C) 2017 Beijing Academy of Food Sciences. Production and hosting by Elsevier B.V.

引用

页码：70 / 76

页数：7

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