Breeding cowpea for resistance to Striga gesnerioides in the Nigerian dry savannas using marker-assisted selection

被引:7
作者
Omoigui, Lucky O. [1 ,2 ,3 ,6 ]
Kamara, Alpha Y. [2 ,3 ]
Moukoumbi, Yonnele D. [2 ,3 ]
Ogunkanmi, Liasu A. [4 ]
Timko, Michael P. [5 ]
机构
[1] Univ Agr Makurdi, Dept Plant Breeding & Seed Sci, PMB 2373, Makurdi, Nigeria
[2] Int Inst Trop Agr, Ibadan, Nigeria
[3] IITA Ltd, Carolym House,26 Dingwall Rd, Croydon CR9 3EE, England
[4] Univ Lagos, Fac Sci, Cell Biol & Genet Dept, PMB 66, Lagos, Nigeria
[5] Univ Virgina, Dept Biol, Charlottesville, VA 22904 USA
[6] IITA, Kano Stn, Ibadan, Nigeria
关键词
Striga gesnerioides; cowpea; resistance; molecular marker; marker-assisted selection; VIGNA-UNGUICULATA L; GENETIC-LINKAGE MAP; SSR MARKERS; ALECTRA; AFLP; INHERITANCE; LINES;
D O I
10.1111/pbr.12475
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Historically, conventional breeding has been the primary strategy used to develop a number of Striga-resistant varieties currently grown in the Sahel of Western Africa. In this study, we have successfully developed and applied a marker-assisted selection strategy that employs a single backcross programme to introgress Striga resistance into farmer preferred varieties of cowpea for the Nigeria savannas. In this strategy, we have introduced the Striga resistance gene from the donor parent IT97K-499-35 into an elite farmer preferred cowpea cultivar Borno Brown'. The selected 47 BC1F2 populations confirmed the recombinants with desirable progeny having Striga resistance gene(s). The 28 lines selected in the BC1F2:4 generation with large seed size, brown seed coat colour and carrying marker alleles were evaluated in the field for resistance to Striga resistance. This led to the selection of a number of desirable improved lines that were immune to Striga having local genetic background with higher yield than those of their parents and standard varieties.
引用
收藏
页码:393 / 399
页数:7
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