Degradation of aflatoxin B1 from naturally contaminated maize using the edible fungus Pleurotus ostreatus

被引:24
作者
Jackson, Lauren W., III [1 ,2 ]
Pryor, Barry M. [1 ]
机构
[1] Univ Arizona, Sch Plant Sci, 1140 E South Campus Dr,POB 210036, Tucson, AZ 85721 USA
[2] Univ Minnesota, Dept Plant Pathol, 1991 Upper Buford Circle, St Paul, MN 55108 USA
来源
AMB EXPRESS | 2017年 / 7卷
关键词
Aflatoxin; Pleurotus ostreatus; Solid-state fermentation; Biodegradation; RICE STRAW; BIODEGRADATION; HEALTH;
D O I
10.1186/s13568-017-0415-0
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aflatoxins are highly carcinogenic secondary metabolites that can contaminate approximately 25% of crops and that cause or exacerbate multiple adverse health conditions, especially in Sub-Saharan Africa and South and Southeast Asia. Regulation and decontamination of aflatoxins in high exposure areas is lacking. Biological detoxification methods are promising because they are assumed to be cheaper and more environmentally friendly compared to chemical alternatives. White-rot fungi produce non-specific enzymes that are known to degrade aflatoxin in in situ and ex situ experiments. The aims of this study were to (1) decontaminate aflatoxin-B-1-(AFB(1)) in naturally contaminated maize with the edible, white-rot fungus Pleurotus ostreatus (oyster mushroom) using a solid-state fermentation system that followed standard cultivation techniques, and to (2) and to assess the risk of mutagenicity in the resulting breakdown products and mushrooms. Vegetative growth and yield characteristics of P. ostreatus were not inhibited by the presence of-AFB(1).-AFB(1) was degraded by up to 94% by the Blue strain. No aflatoxin could be detected in P. ostreatus mushrooms produced from-AFB(1)-contaminated maize. Moreover, the mutagenicity of breakdown products from the maize substrate, and reversion of breakdown products to the parent compound, were minimal. These results suggest that P. ostreatus significantly degrades-AFB(1) in naturally contaminated maize under standard cultivation techniques to levels that are acceptable for some livestock fodder, and that using P. ostreatus to bioconvert crops into mushrooms can reduce-AFB(1)-related losses.
引用
收藏
页码:1 / 7
页数:7
相关论文
共 22 条
  • [1] The biodegradation of wheat straw by Pleurotus ostreatus mushrooms and its use in cattle feeding
    Adamovic, M
    Grubic, G
    Milenkovic, I
    Jovanovic, R
    Protic, R
    Sretenovic, L
    Stoicevic, L
    [J]. ANIMAL FEED SCIENCE AND TECHNOLOGY, 1998, 71 (3-4) : 357 - 362
  • [2] Degradation of aflatoxin B1 by fungal laccase enzymes
    Alberts, J. F.
    Gelderblom, W. C. A.
    Botha, A.
    van Zyl, W. H.
    [J]. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2009, 135 (01) : 47 - 52
  • [3] METHODS FOR DETECTING CARCINOGENS AND MUTAGENS WITH SALMONELLA-MAMMALIAN-MICROSOME MUTAGENICITY TEST
    AMES, BN
    MCCANN, J
    YAMASAKI, E
    [J]. MUTATION RESEARCH, 1975, 31 (06): : 347 - 363
  • [4] Cotty P.J., 2008, Mycotoxins: Detection methods, management, public health and agricultural trade
  • [5] ZINNIOL PRODUCTION BY ALTERNARIA SPECIES
    COTTY, PJ
    MISAGHI, IJ
    [J]. PHYTOPATHOLOGY, 1984, 74 (07) : 785 - 788
  • [6] Cotty PJ, 1994, GENUS ASPERGILLUS
  • [7] Aflatoxin B1 degradation during co-cultivation of Aspergillus flavus and Pleurotus ostreatus strains on rice straw
    Das, Arijit
    Bhattacharya, Sourav
    Palaniswamy, Muthusamy
    Angayarkanni, Jayaraman
    [J]. 3 BIOTECH, 2015, 5 (03) : 279 - 284
  • [8] Biodegradation of aflatoxin B1 in contaminated rice straw by Pleurotus ostreatus MTCC 142 and Pleurotus ostreatus GHBBF10 in the presence of metal salts and surfactants
    Das, Arijit
    Bhattacharya, Sourav
    Palaniswamy, Muthusamy
    Angayarkanni, Jayaraman
    [J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2014, 30 (08) : 2315 - 2324
  • [9] BASE SUBSTITUTION MUTATIONS INDUCED BY METABOLICALLY ACTIVATED AFLATOXIN-B1
    FOSTER, PL
    EISENSTADT, E
    MILLER, JH
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (09): : 2695 - 2698
  • [10] OpenCFU, a New Free and Open-Source Software to Count Cell Colonies and Other Circular Objects
    Geissmann, Quentin
    [J]. PLOS ONE, 2013, 8 (02):