Development of glucose biosensor based on reconstitution of glucose oxidase onto polymeric redox mediator coated pencil graphite electrodes

被引：29

作者：

Dervisevic, Muamer ^{[1
]}

Cevik, Emre ^{[1
]}

Senel, Mehmet ^{[2
]}

机构：

[1] Fatih Univ, Fac Engn, Dept Genet & Bioengn, TR-34500 Istanbul, Turkey

[2] Fatih Univ, Fac Arts & Sci, Dept Chem, TR-34500 Istanbul, Turkey

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2015年 / 68卷

关键词：

Glucose; Mediator; Ferrocene; Amperometric biosensor; FLAVIN-ADENINE-DINUCLEOTIDE; AMPEROMETRIC BIOSENSORS; COVALENT IMMOBILIZATION; HORSERADISH-PEROXIDASE; NANOPARTICLES; FERROCENE; HOMOPOLYMER; COPOLYMERS; MONOLAYER; PLATINUM;

D O I：

10.1016/j.enzmictec.2014.09.007

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this study, a novel glucose biosensor was fabricated by reconstitutional immobilization of glucose oxidase (GOx) onto a poly(glycidyl methacrylate-co-vinylferrocene) (poly(GMA-co-VFc)) film coated pencil graphite electrode (PGE). The amperometric current response of poly(GMA-co-VFc)-GOx to glucose is linear in the concentration range between 1 and 16 mM (correlation coefficient of 0.9998) with a detection limit of 2.7 mu M (S/N=3). Experimental parameters were studied in detail and optimized, including the pH and temperature governing the analytical performance of the biosensor. The stability and reusability of the biosensor as well as its kinetic parameters have also been studied. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：69 / 76

页数：8

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