A glutathione s-transferase confers herbicide tolerance in rice

被引:15
作者
Hu, Tingzhang [1 ]
机构
[1] Chongqing Three Gorges Univ, Sch Life Sci & Engn, Chongqing 404000, Peoples R China
来源
CROP BREEDING AND APPLIED BIOTECHNOLOGY | 2014年 / 14卷 / 02期
关键词
Oryza sativa L; glutathione S-transferase; over-expression; herbicide resistance; FUNCTIONAL DIVERGENCE; EXPRESSION; PLANTS; PROTEIN; GENE; RESISTANCE; EVOLUTION; STRESS; ROLES;
D O I
10.1590/1984-70332014v14n2a14
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Plant glutathione S-transferases (GSTs) have been a focus of attention due to their role in herbicide detoxification. OsGSTL2 is a glutathione S-transferase, lambda class gene from rice (Oryza sativa L.). Transgenic rice plants over-expressing OsGSTL2 were generated from rice calli by the use of an Agrobacterium transformation system, and were screened by a combination of hygromycin resistance, PCR and Southern blot analysis. In the vegetative tissues of transgenic rice plants, the over-expression of OsGSTL2 not only increased levels of OsGSTL2 transcripts, but also GST and GPX expression, while reduced superoxide. Transgenic rice plants also showed higher tolerance to glyphosate and chlorsulfuron, which often contaminate agricultural fields. The findings demonstrate the detoxification role of OsGSTL2 in the growth and development of rice plants. It should be possible to apply the present results to crops for developing herbicide tolerance and for limiting herbicide contamination in the food chain.
引用
收藏
页码:76 / 81
页数:6
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