Real-time PCR carried out on DNA extracted from serum or blood sample is not a good method for surveillance of bovine brucellosis

被引：8

作者：

Tiwari, Arvind ^{[1
]}

Pal, Vijai ^{[1
]}

Afley, Prachiti ^{[1
]}

Sharma, Deepak Kumar ^{[2
]}

Bhatnagar, Chandra Shekhar ^{[2
]}

Bhardwaj, Bhupendra ^{[2
]}

Rai, Ganga Prasad ^{[1
]}

Kumar, Subodh ^{[1
]}

机构：

[1] Def Res & Dev Estab, Div Microbiol, Gwalior 474002, Madhya Pradesh, India

[2] Dept Anim Husb, Reg Dis Diagnost Ctr, Udaipur 313001, Rajasthan, India

来源：

TROPICAL ANIMAL HEALTH AND PRODUCTION | 2014年 / 46卷 / 08期

关键词：

Brucella abortus; Brucellosis; Disease surveillance; Real-time PCR; Serodiagnosis; POLYMERASE-CHAIN-REACTION; LINKED-IMMUNOSORBENT-ASSAY; DIAGNOSIS; ABORTUS; INDIA;

D O I：

10.1007/s11250-014-0664-8

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Bovine brucellosis is endemic in many parts of the world including India. The disease diagnosis and surveillance are usually carried out by serological tests, which however have drawbacks. This study was undertaken to evaluate the potential of real-time PCR (RT-PCR) targeting bcsp31 gene for surveillance of bovine brucellosis. A total of 461 samples, which included 408 stored serum and 53 prospective blood samples, were used. It was found that 33 (7.15 %) samples were positive by RT-PCR, whereas 149 (32.32 %) and 132 (28.63 %) were positive by Rose Bengal plate test (RBPT) or standard agglutination test (STAT), respectively. The results of this study suggest that RT-PCR targeting bcsp31 gene carried out on DNA extracted from serum or blood may not be a suitable method for surveillance of brucellosis in bovines.

引用

页码：1519 / 1522

页数：4

共 20 条

[1]

Adone R, 2013, REV SCI TECH OIE, V32, P199

[2] Comparison of Different PCR Methods for Detection of Brucella spp. in Human Blood Samples [J].

Al-Ajlan, Hisham H. ;

Ibrahim, Abdelnasser S. S. ;

Al-Salamah, Ali A. .

POLISH JOURNAL OF MICROBIOLOGY, 2011, 60 (01) :27-33

[3] Interactions of the Human Pathogenic Brucella Species with Their Hosts [J].

Atluri, Vidya L. ;

Xavier, Mariana N. ;

de Jong, Maarten F. ;

den Hartigh, Andreas B. ;

Tsolis, Renee M. .

ANNUAL REVIEW OF MICROBIOLOGY, VOL 65, 2011, 65 :523-541

[4]

Baddour MM, 2008, CAN J MICROBIOL, V54, P352, DOI [10.1139/W08-017, 10.1139/w08-017]

[5]

BAILY GG, 1992, J TROP MED HYG, V95, P271

[6]

CORBEL MJ, 1984, DEV BIOLOGICALS, V56, P341

[7] Detection of Brucella DNA in sera from patients with brucellosis by polymerase chain reaction [J].

Elfaki, MG ;

Uz-Zaman, T ;

Al-Hokail, AA ;

Nakeeb, SM .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2005, 53 (01) :1-7

[8] How to substantiate eradication of bovine brucellosis when aspecific serological reactions occur in the course of brucellosis testing [J].

Godfroid, J ;

Saegerman, C ;

Wellemans, V ;

Walravens, K ;

Letesson, JJ ;

Tibor, A ;

Mc Millan, A ;

Spencer, S ;

Sanna, M ;

Bakker, D ;

Pouillot, R ;

Garin-Bastuji, B .

VETERINARY MICROBIOLOGY, 2002, 90 (1-4) :461-477

[9] Comparison of diagnostic tests for the detection of Brucella spp. in camel sera [J].

Gwida M.M. ;

El-Gohary A.H. ;

Melzer F. ;

Tomaso H. ;

Rösler U. ;

Wernery U. ;

Wernery R. ;

Elschner M.C. ;

Khan I. ;

Eickhoff M. ;

Schöner D. ;

Neubauer H. .

BMC Research Notes, 4 (1)

[10]

Leal-Klevezas D S, 1995, Arch Med Res, V26, P263

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