The mycelium of the Trametes versicolor (Turkey tail) mushroom and its fermented substrate each show potent and complementary immune activating properties in vitro

被引:41
作者
Benson, Kathleen F. [1 ]
Stamets, Paul [2 ]
Davis, Renee [2 ]
Nally, Regan [2 ]
Taylor, Alex [2 ]
Slater, Sonya [2 ]
Jensen, Gitte S. [1 ]
机构
[1] NIS Labs, 1437 Esplanade, Klamath Falls, OR 97601 USA
[2] Fungi Perfecti, Postal Box 7634, Olympia, WA 98507 USA
来源
BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2019年 / 19卷 / 01期
关键词
Anti-inflammatory; Immune modulation; CD69; Cytokines; NATURAL-KILLER-CELLS; VERA-BASED EXTRACT; HUMAN NK-CELLS; STRUCTURAL CHARACTERISTICS; ANTIOXIDANT ACTIVITY; POLYSACCHARIDE-K; YEAST CULTURE; UP-REGULATION; WHOLE-BLOOD; IFN-GAMMA;
D O I
10.1186/s12906-019-2681-7
中图分类号
R [医药、卫生];
学科分类号
10 ;
摘要
Background The medicinal mushroom Trametes versicolor (Tv, Turkey Tail) is often prepared for consumption as a powder from the fungal mycelium and the fermented substrate on which it grew. The goal for this study was to evaluate the immune-modulating properties of the mycelium versus the fermented substrate, to document whether an important part of the immune-activating effects resides in the metabolically fermented substrate. Methods Tv mycelium was cultured on rice flour. The mycelium and the fermented substrate were mechanically separated, dried, and milled. The initial substrate served as a control. Aqueous fractions were extracted and passed through 0.22-mu m filters. The remaining solids were passed through homogenization spin columns without filtration. The aqueous and solid fractions of the initial substrate (IS), the fermented substrate (FS), and the Trametes versicolor mycelium (TvM) were tested for immune-activating and modulating activities on human peripheral blood mononuclear cell cultures, to examine expression of the CD69 activation marker on lymphocytes versus monocytes, and on the T, NKT, and NK lymphocyte subsets. Culture supernatants were tested for cytokines using Luminex arrays. Results Both aqueous and solid fractions of TvM triggered robust induction of CD69 on lymphocytes and monocytes, whereas FS only triggered minor induction of CD69, and IS had no activating effect. The aqueous extract of TvM had stronger activating effects than the solid fraction. In contrast, the solid fraction of IS triggered a reduction in CD69, below levels on untreated cells. Both aqueous and solid fractions of FS triggered large and dose-dependent increases in immune-activating pro-inflammatory cytokines (IL-2, IL-6), anti-inflammatory cytokines Interleukin-1 receptor antagonist (IL-1ra) and Interleukin-10 (IL-10), anti-viral cytokines interferon-gamma (IFN-gamma) and Macrophage Inflammatory Protein-alpha (MIP-1 alpha), as well as Granulocyte-Colony Stimulating Factor (G-CSF) and Interleukin-8 (IL-8). TvM triggered more modest cytokine increases. The aqueous extract of IS showed no effects, whereas the solid fraction showed modest effects on induction of cytokines and growth factors. Conclusion The results demonstrated that the immune-activating bioactivity of a mycelial-based medicinal mushroom preparation is a combination of the mycelium itself (including insoluble beta-glucans, and also water-soluble components), and the highly bioactive, metabolically fermented substrate, not present in the initial substrate.
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页数:14
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共 62 条
  • [1] Anti-inflammatory and anti-tumor-promoting effects of triterpene acids and sterols from the fungus Ganoderma lucidum
    Akihisa, Toshihiro
    Nakamura, Yuji
    Tagata, Masaaki
    Tokuda, Harukuni
    Yasukawa, Ken
    Uchiyama, Emiko
    Suzuki, Takashi
    Kimura, Yumiko
    [J]. CHEMISTRY & BIODIVERSITY, 2007, 4 (02) : 224 - 231
  • [2] Aung S.K., 2005, International Journal of Medical Mushrooms, V7, P375, DOI DOI 10.1615/INTJMEDMUSHROOMS.V7.I3.290
  • [3] Determination of Glucan Contents in the Fruiting Bodies and Mycelia of Lentinula edodes Cultivars
    Bak, Won Chull
    Park, Ji Heon
    Park, Young Ae
    Ka, Kang Hyeon
    [J]. MYCOBIOLOGY, 2014, 42 (03) : 301 - 304
  • [4] Medicinal Mushroom Lingzhi or Reishi, Ganoderma lucidum (W.Curt.:Fr.) P. Karst., β-Glucan Induces Toll-like Receptors and Fails to Induce Inflammatory Cytokines in NF-κB Inhibitor-Treated Macrophages
    Batbayar, Sainkhuu
    Kim, Mi Jeong
    Kim, Ha Won
    [J]. INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS, 2011, 13 (03) : 213 - 225
  • [5] Human NK Cell Up-regulation of CD69, HLA-DR, Interferon γ Secretion and Cytotoxic Activity by Plasmacytoid Dendritic Cells is Regulated through Overlapping but Different Pathways
    Benlahrech, Adel
    Donaghy, Heather
    Rozis, George
    Goodier, Martin
    Klavinskis, Linda
    Gotch, Frances
    Patterson, Steven
    [J]. SENSORS, 2009, 9 (01): : 386 - 403
  • [6] Water-soluble egg membrane enhances the immunoactivating properties of an Aloe vera-based extract of Nerium oleander leaves
    Benson, Kathleen F.
    Newman, Robert A.
    Jensen, Gitte S.
    [J]. CLINICAL COSMETIC AND INVESTIGATIONAL DERMATOLOGY, 2016, 9 : 393 - 403
  • [7] Antioxidant, anti-inflammatory, anti-apoptotic, and skin regenerative properties of an Aloe vera-based extract of Nerium oleander leaves (NAE-8 (R))
    Benson, Kathleen F.
    Newman, Robert A.
    Jensen, Gitte S.
    [J]. CLINICAL COSMETIC AND INVESTIGATIONAL DERMATOLOGY, 2015, 8 : 239 - 248
  • [8] West African Sorghum bicolor Leaf Sheaths Have Anti-Inflammatory and Immune-Modulating Properties In Vitro
    Benson, Kathleen F.
    Beaman, Joni L.
    Ou, Boxin
    Okubena, Ademola
    Okubena, Olajuwon
    Jensen, Gitte S.
    [J]. JOURNAL OF MEDICINAL FOOD, 2013, 16 (03) : 230 - 238
  • [9] A novel extract from bovine colostrum whey supports anti-bacterial and anti-viral innate immune functions in vitro and in vivo I. Enhanced immune activity in vitro translates to improved microbial clearance in animal infection models
    Benson, Kathleen F.
    Carter, Steve G.
    Patterson, Kelly M.
    Patel, Dilip
    Jensen, Gitte S.
    [J]. PREVENTIVE MEDICINE, 2012, 54 : S116 - S123
  • [10] Purification and preliminary crystallographic study of Trametes versicolor laccase in its native form
    Bertrand, T
    Jolivalt, C
    Caminade, E
    Joly, N
    Mougin, C
    Briozzo, P
    [J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2002, 58 : 319 - 321