Secretory expression of a heterologous nattokinase in Lactococcus lactis

被引：66
作者：
Liang, Xiaobo
Zhang, Lixin
Zhong, Jin [1 ]
Huan, Liandong
机构：
[1] Chinese Acad Sci, Inst Microbiol, Ctr Metab Engn Microorganisms, Beijing 100080, Peoples R China
[2] Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resource, Beijing 100080, Peoples R China
[3] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
[4] Chinese Acad Sci, Sea Inst Oceanol, Guangzhou 510301, Peoples R China
来源：
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2007年 / 75卷 / 01期
关键词：
D O I：
10.1007/s00253-006-0809-4
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Nattokinase has been reported as an oral health product for the prevention of atherosclerosis. We developed a novel strategy to express a nattokinase from Bacillus subtilis in a live delivery vehicle, Lactococcus lactis. Promoter P (nisZ) and signal peptide SPUsp were used for inducible and secretory expression of nattokinase in L. lactis. Western blotting analysis demonstrated that nattokinase was successfully expressed, and about 94% of the enzyme was secreted to the culture. The recombinant nattokinase showed potent fibrinolytic activity, equivalent to 41.7 urokinase units per milliliter culture. Expression and delivery of such a fibrinolytic enzyme in the food-grade vehicle L. lactis would facilitate the widespread application of nattokinase in the control and prevention of thrombosis diseases.
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页码：95 / 101
页数：7
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