A R2R3 MYB transcription factor from ash positively regulates salt response in tobacco

被引：0
作者：
Li, Tian [1 ,2 ]
Sun, Jingkuan [1 ]
Bi, Yuping [2 ]
机构：
[1] Binzhou Univ, Shandong Prov Key Lab Ecoenvironm Sci Yellow Riv, Binzhou, Shandong, Peoples R China
[2] Shandong Acad Agr Sci, Biotechnol Res Ctr, Shandong Prov Key Lab Crop Genet Improvement Ecol, Jinan, Shandong, Peoples R China
来源：
TURKISH JOURNAL OF BIOLOGY | 2017年 / 41卷 / 04期
基金：
中国国家自然科学基金;
关键词：
Ash; MYB transcription factor; transcription; salt stress; ARABIDOPSIS-THALIANA; DROUGHT TOLERANCE; ABIOTIC STRESS; FACTOR GENE; MOLECULAR CHARACTERIZATION; DEHYDRATION TOLERANCE; FUNCTIONAL-ANALYSIS; TRANSGENIC TOBACCO; ACID BIOSYNTHESIS; OVER-EXPRESSION;
D O I：
10.3906/biy-1612-31
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The roles of MYB transcription factors are diverse and important in regulating environmental stress in plants. In this study, the R2R3 MYB gene FvMYB2 (GenBank Accession No. KY767843) and its promoter region were cloned from ash (Fraxinus velutina Torr.). Phylogenetic analysis revealed that FvMYB2 was clustered with VvMYB60 from Vitis vinifera. PlantCARE software analysis showed that its promoter contained different cis elements involved in diverse abiotic stresses. The expression patterns of FvMYB2 were investigated under different abiotic stress conditions. FvMYB2 subcellular localization was mainly localized in the nucleus. Overexpression of FvMYB2 in tobacco showed enhanced salt resistance and upregulation of stress-and ABA-related genes after NaCl treatment. These results indicated that FvMYB2 may play a positive role in salt stress regulation mediated by ABA-dependent signaling mechanisms.
引用
收藏
页码：608 / 621
页数：14
相关论文
共 57 条
  • [1] Transcription factors in plants and ABA dependent and independent abiotic stress signalling
    Agarwal, P. K.
    Jha, B.
    [J]. BIOLOGIA PLANTARUM, 2010, 54 (02) : 201 - 212
  • [2] MYB transcription factor genes as regulators for plant responses: an overview
    Ambawat, Supriya
    Sharma, Poonam
    Yadav, Neelam R.
    Yadav, Ram C.
    [J]. PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS, 2013, 19 (03) : 307 - 321
  • [3] Genomic and transcriptomic characterization of the transcription factor family R2R3-MYB in soybean and its involvement in the resistance responses to Phakopsora pachyrhizi
    Aoyagi, Luciano N.
    Lopes-Caitar, Valeria S.
    de Carvalho, Mayra C. C. G.
    Darben, Luana M.
    Polizel-Podanosqui, Adriana
    Kuwahara, Marcia K.
    Nepomuceno, Alexandre L.
    Abdelnoor, Ricardo V.
    Marcelino-Guimaraes, Francismar C.
    [J]. PLANT SCIENCE, 2014, 229 : 32 - 42
  • [4] Isolation and functional analysis of a MYB transcription factor gene that is a key regulator for the development of red coloration in apple skin
    Ban, Yusuke
    Honda, Chikako
    Hatsuyama, Yoshimichi
    Igarashi, Megumi
    Bessho, Hideo
    Moriguchi, Takaya
    [J]. PLANT AND CELL PHYSIOLOGY, 2007, 48 (07) : 958 - 970
  • [5] The effect of drought and heat stress on reproductive processes in cereals
    Barnabas, Beata
    Jaeger, Katalin
    Feher, Attila
    [J]. PLANT CELL AND ENVIRONMENT, 2008, 31 (01) : 11 - 38
  • [6] The tomato mutant ars1 (altered response to salt stress 1) identifies an R1-type MYB transcription factor involved in stomatal closure under salt acclimation
    Campos, Juan F.
    Cara, Beatriz
    Perez-Martin, Fernando
    Pineda, Benito
    Egea, Isabel
    Flores, Francisco B.
    Fernandez-Garcia, Nieves
    Capel, Juan
    Moreno, Vicente
    Angosto, Trinidad
    Lozano, Rafael
    Bolarin, Maria C.
    [J]. PLANT BIOTECHNOLOGY JOURNAL, 2016, 14 (06) : 1345 - 1356
  • [7] Over-expression of OsDREB genes lead to enhanced drought tolerance in rice
    Chen, Jian-Qiang
    Meng, Xiu-Ping
    Zhang, Yun
    Xia, Mian
    Wang, Xi-Ping
    [J]. BIOTECHNOLOGY LETTERS, 2008, 30 (12) : 2191 - 2198
  • [8] Isolation and characterization of genes encoding Myb transcription factor in wheat (Triticum aestivem L.)
    Chen, RM
    Ni, ZF
    Nie, XL
    Qin, YX
    Dong, GQ
    Sun, QX
    [J]. PLANT SCIENCE, 2005, 169 (06) : 1146 - 1154
  • [9] Overexpression of sheepgrass R1-MYB transcription factor LcMYB1 confers salt tolerance in transgenic Arabidopsis
    Cheng, Liqin
    Li, Xiaoxia
    Huang, Xin
    Ma, Tian
    Liang, Ye
    Ma, Xingyong
    Peng, Xianjun
    Jia, Junting
    Chen, Shuangyan
    Chen, Yan
    Deng, Bo
    Liu, Gongshe
    [J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2013, 70 : 252 - 260
  • [10] Dong XF, 2012, INT J AGRIC BIOL, V14, P575
123456