Genetic Analysis and Molecular Mapping of a Novel Gene Conferring Resistance to Rice Stripe Virus

被引：15

作者：

Zhao, Feng ^{[1
,4
]}

Cai, Zhijun ^{[2
]}

Hu, Tiezhu ^{[3
]}

Yao, Haigen ^{[2
]}

Wang, Li ^{[1
]}

Dong, Na ^{[3
]}

Wang, Bin ^{[1
]}

Ru, Zhengang ^{[3
]}

Zhai, Wenxue ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China

[2] Jiaxing Acad Agr Sci, Jiaxing 314000, Peoples R China

[3] Coll Sci & Technol Henan Prov, Xinxiang 453003, Peoples R China

[4] Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai 201306, Peoples R China

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 2010年 / 28卷 / 03期

关键词：

Rice stripe resistance gene RSV1; Gene mapping; SSR and SRAP markers; SMALL BROWN PLANTHOPPER; DISEASE-RESISTANCE; BREEDING PROGRAMS; LAODELPHAX-STRIATELLUS; INTROGRESSION; MARKERS; XA21; PCR;

D O I：

10.1007/s11105-009-0178-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Rice stripe virus (RSV) is one of the most damaging diseases affecting rice in East Asia. Rice variety 502 is highly resistant to RSV, while variety 5112 is extremely susceptible. Field statistical data revealed that all "502 x 5112" F-1 individuals were resistant to RSV and the ratio of resistant to susceptible plants was 3:1 in the F-2 population and 1:1 in the BC1F1 population. These results indicated that a dominant gene, designated RSV1, controlled the resistance. Simple sequence repeat (SSR) analysis was subsequently carried out in an F-2 population. Sixty SSR markers evenly distributed on the 12 rice chromosomes were screened and tested. Two markers, RM229 and RM206, showed linkage with RSV1. Based on this result, six SSR markers flanking RM229 and RM206 were further selected and tested. Results indicated that SSR markers RM457 and RM473E were linked to RSV1 with a genetic distance of 4.5 and 5.0 cM, respectively. All of the four SSR markers (RM229, RM473E, RM457 and RM206) linked to RSV1 were all located on chromosome 11, therefore RSV1 should be located on chromosome 11 also. In order to find some new markers more closely linked to the RSV1 gene, sequence-related amplified polymorphism (SRAP) analysis was performed. A total of 30 SRAP primer-pairs were analyzed, and one marker SR1 showed linkage with RSV1 at a genetic distance of 2.9 cM. Finally, RSV1 gene was mapped on chromosome 11 between SSR markers RM457 and SRAP marker SR1 with a genetic distance of 4.5 cM and 2.9 cM, respectively.

引用

页码：512 / 518

页数：7

共 31 条

[1]

[Anonymous], 1985, COMMONWEALTH MYCOLOG

[2] Detecting Rice stripe virus (RSV) in the small brown planthopper (Laodelphax striatellus) with high specificity by RT-PCR [J].

Cai, LJ ;

Ma, XZ ;

Kang, L ;

Deng, KJ ;

Zhao, SY ;

Li, CB .

JOURNAL OF VIROLOGICAL METHODS, 2003, 112 (1-2) :115-120

[3]

DING XL, 2005, ACTA AGRONOMICA SINI, V319, P1041

[4] Localization of the rice stripe disease resistance gene, Stv-bi, by graphical genotyping and linkage analyses with molecular markers [J].

Hayano-Saito, Y ;

Tsuji, T ;

Fujii, K ;

Saito, K ;

Iwasaki, M ;

Saito, A .

THEORETICAL AND APPLIED GENETICS, 1998, 96 (08) :1044-1049

[5]

HILLEL J, 1990, GENETICS, V124, P783

[6]

HOSPITAL F, 1992, GENETICS, V132, P1199

[7] Molecular tagging of a gene for resistance to brown planthopper in rice (Oryza sativa L.) [J].

Jena, KK ;

Pasalu, IC ;

Rao, YK ;

Varalaxmi, Y ;

Krishnaiah, K ;

Khush, GS ;

Kochert, G .

EUPHYTICA, 2003, 129 (01) :81-88

[8]

LANDER E S, 1987, Genomics, V1, P174, DOI 10.1016/0888-7543(87)90010-3

[9] Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction:: its application to mapping and gene tagging in Brassica [J].

Li, G ;

Quiros, CF .

THEORETICAL AND APPLIED GENETICS, 2001, 103 (2-3) :455-461

[10] Application of microsatellites from maize to teosinte and other relatives of maize [J].

Lübberstedt, T ;

Dussle, C ;

Melchinger, AE .

PLANT BREEDING, 1998, 117 (05) :447-450

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