Phosphorylation Modulates the Subcellular Localization of SOX11

被引:18
作者
Balta, Elli-Anna [1 ]
Wittmann, Marie-Theres [1 ]
Jung, Matthias [1 ]
Sock, Elisabeth [1 ]
Haeberle, Benjamin Martin [1 ]
Heim, Birgit [2 ]
von Zweydorf, Felix [3 ]
Heppt, Jana [1 ]
von Wittgenstein, Julia [1 ]
Gloeckner, Christian Johannes [2 ,3 ]
Lie, Dieter Chichung [1 ]
机构
[1] Friedrich Alexander Univ Erlangen Numberg, Inst Biochem, Erlangen, Germany
[2] Univ Tubingen, Ctr Ophthalmol, Inst Ophthalm Res, Tubingen, Germany
[3] DZNE German Ctr Neurodegenerat Dis, Tubingen, Germany
来源
FRONTIERS IN MOLECULAR NEUROSCIENCE | 2018年 / 11卷
基金
奥地利科学基金会;
关键词
SOX11; transcription factor phosphorylation; subcellular localization; neurogenesis; cancer; intellectual disability; TRANSCRIPTION FACTOR SOX11; ADULT HIPPOCAMPAL NEUROGENESIS; MANTLE CELL LYMPHOMA; EXPRESSION; PROTEIN; REVEALS; DIFFERENTIATION; MAINTENANCE; INDUCTION; SUGGESTS;
D O I
10.3389/fnmol.2018.00211
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
SOX11 is a key Transcription Factor (TF) in the regulation of embryonic and adult neurogenesis, whose mutation has recently been linked to an intellectual disability syndrome in humans. SOX11's transient activity during neurogenesis is critical to ensure the precise execution of the neurogenic program. Here, we report that SOX11 displays differential subcellular localizations during the course of neurogenesis. Western-Blot analysis of embryonic mouse brain lysates indicated that SOX11 is post-translationally modified by phosphorylation. Using Mass Spectrometry, we found 10 serine residues in the SOX11 protein that are putatively phosphorylated. Systematic analysis of phospho-mutant SOX11 resulted in the identification of the S30 residue, whose phosphorylation promotes nuclear over cytoplasmic localization of SOX11. Collectively, these findings uncover phosphorylation as a novel layer of regulation of the intellectual disability gene Sox11.
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页数:13
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