Use of extracellular matrix hydrogel from human placenta to restore hair-inductive potential of dermal papilla cells

被引：38

作者：

Zhang, Xinyu ^{[1
,2
]}

Xiao, Shune ^{[2
]}

Liu, Bingcheng ^{[2
]}

Miao, Yong ^{[2
]}

Hu, Zhiqi ^{[2
]}

机构：

[1] Xi An Jiao Tong Univ, Affiliated Hosp 1, Dept Plast Cosmet & Maxillofacial Surg, Xian, Shaanxi, Peoples R China

[2] Southern Med Univ, Nan Fang Hosp, Dept Plast Surg, Guangzhou, Guangdong, Peoples R China

来源：

REGENERATIVE MEDICINE | 2019年 / 14卷 / 08期

关键词：

3D culture; decellularization; dermal papilla cell; extracellular matrix; hair follicle regeneration; hair loss; human placenta; hydrogel; thermosensitive; tissue-engineering; ANDROGENETIC ALOPECIA; IN-VITRO; GROWTH; MORPHOGENESIS; SPHEROIDS; MINOXIDIL; LIVER;

D O I：

10.2217/rme-2018-0112

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Aim: To explore the feasibility of human placenta extracellular matrix (HPECM) hydrogel in restoring the hair-inductive capacity of high-passaged (P8) dermal papilla cells (DPCs) for hair follicle regeneration. Materials & methods: HPECM hydrogel was prepared following decellularization and enzymatic solubilization treatment. DPCs isolated from human scalp were cultured in 2D and 3D environments. The hairinductive ability of DPCs was assessed by quantitative RT-PCR, immunofluorescence staining, immunoblotting and patch assay. Results: DPCs (P8) formed spheres when cultured on the HPECM hydrogel. The expression levels of Versican, ALP, and beta-catenin were restored in the DP spheres. HPECM hydrogel-cultured DP spheres co-grafted with newborn mouse epidermal cells regenerated new hair follicle. Conclusion: HPECM hydrogel successfully restores the hair-inductive capacity of high-passaged DPCs.

引用

页码：741 / 751

页数：11

共 55 条

[1] Characterization of the Epicardial Adipose Tissue in Decellularized Human-Scaled Whole Hearts: Implications for the Whole-Heart Tissue Engineering [J].

Akhyari, Payam ;

Oberle, Fabian ;

Huelsmann, Joern ;

Heid, Hans ;

Lehr, Stefan ;

Barbian, Andreas ;

Nakanishi, Sentaro ;

Aubin, Hug ;

Jenke, Alexander ;

Lichtenberg, Artur .

TISSUE ENGINEERING PART A, 2018, 24 (7-8) :682-693

[2]

[Anonymous], 2017, DERMATOL ONLINE J

[3] Extracellular matrix as a biological scaffold material: Structure and function [J].

Badylak, Stephen F. ;

Freytes, Donald O. ;

Gilbert, Thomas W. .

ACTA BIOMATERIALIA, 2009, 5 (01) :1-13

[4] The use of minoxidil in the treatment of male and female androgenetic alopecia: a story of more than 30 years [J].

Barbareschi, Mauro .

GIORNALE ITALIANO DI DERMATOLOGIA E VENEREOLOGIA, 2018, 153 (01) :102-106

[5] Matrigel: From discovery and ECM mimicry to assays and models for cancer research [J].

Benton, Gabriel ;

Arnaoutova, Irina ;

George, Jay ;

Kleinman, Hynda K. ;

Koblinski, Jennifer .

ADVANCED DRUG DELIVERY REVIEWS, 2014, 79-80 :3-18

[6]

Chen GB, 2018, METHODS MOL BIOL, V1577, P239, DOI 10.1007/7651_2017_50

[7]

Choi JS, 2013, TISSUE ENG PT A, V19, P329, DOI [10.1089/ten.TEA.2011.0738, 10.1089/ten.tea.2011.0738]

[8] Tissue Engineering of Rat Bladder Using Marrow-Derived Mesenchymal Stem Cells and Bladder Acellular Matrix [J].

Coutu, Daniel L. ;

Mahfouz, Wally ;

Loutochin, Oleg ;

Galipeau, Jacques ;

Corcos, Jacques .

PLOS ONE, 2014, 9 (12)

[9] Clonal Growth of Dermal Papilla Cells in Hydrogels Reveals Intrinsic Differences between Sox2-Positive and -Negative Cells In Vitro and In Vivo [J].

Driskell, Ryan R. ;

Juneja, Vikram R. ;

Connelly, John T. ;

Kretzschmar, Kai ;

Tan, David W. -M. ;

Watt, Fiona M. .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2012, 132 (04) :1084-1093

[10] Hair follicle dermal papilla cells at a glance [J].

Driskell, Ryan R. ;

Clavel, Carlos ;

Rendl, Michael ;

Watt, Fiona M. .

JOURNAL OF CELL SCIENCE, 2011, 124 (08) :1179-1182

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