Intramuscular Matrix-M-adjuvanted virosomal H5N1 vaccine induces high frequencies of multifunctional Th1 CD4+ cells and strong antibody responses in mice

被引：47
作者：
Madhun, Abdullah S. [1 ]
Haaheim, Lars R. [1 ]
Nilsen, Mona V. [1 ]
Cox, Rebecca J. [1 ]
机构：
[1] Univ Bergen, Gade Inst, Influenza Ctr, N-5021 Bergen, Norway
来源：
VACCINE | 2009年 / 27卷 / 52期
关键词：
Multifunctional Th1; Matrix-M; Virosomal; Influenza; CYTOTOXIC T-LYMPHOCYTE; INFLUENZA-VIRUS INFECTION; CROSS-PROTECTION; MF59-ADJUVANTED INFLUENZA; ISCOMATRIX(TM) ADJUVANT; CANDIDATE VACCINES; IMMUNE-RESPONSES; RANDOMIZED-TRIAL; LETHAL CHALLENGE; ISCOM VACCINE;
D O I：
10.1016/j.vaccine.2009.09.044
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Ideally, a candidate pandemic influenza vaccine should elicit rapid and strong cell-mediated and humoral immune responses, which are long-lasting and exhibit broad cross-reactivity against drifted strains. The present study investigated the detailed humoral and cellular immune responses in mice vaccinated intranasally or intramuscularly with inactivated influenza H5N1 (NIBRG-14) virosomal vaccine alone or formulated with Matrix-M adjuvant. The intramuscular Matrix-M-adjuvanted vaccine induced a strong immediate and long-term humoral immune response with high cross-reactivity against drifted H5N1 viruses and showed a close-sparing potential. Additionally, the vaccine induced a balanced Th1/Th2 cytokine profile and most importantly high frequencies of multifunctional Th1 CD4(+) cells. Our results highlight that Matrix-M adjuvant is a promising parenteral adjuvant for formulating pandemic candidate vaccines. (C) 2009 Elsevier Ltd. All rights reserved
引用
收藏
页码：7367 / 7376
页数：10
相关论文
共 60 条
  • [11] Non-lethal viral challenge of influenza haemagglutinin and nucleoprotein DNA vaccinated mice results in reduced viral replication
    Cox, RJ
    Mykkeltvedt, E
    Robertson, J
    Haaheim, LR
    [J]. SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 2002, 55 (01) : 14 - 23
  • [12] Virosomes for antigen and DNA delivery
    Daemen, T
    de Mare, A
    Bungener, L
    de Jonge, J
    Huckriede, A
    Wilschut, J
    [J]. ADVANCED DRUG DELIVERY REVIEWS, 2005, 57 (03) : 451 - 463
  • [13] Multifunctional TH1 cells define a correlate of vaccine-mediated protection against Leishmania major
    Darrah, Patricia A.
    Patel, Dipti T.
    De Luca, Paula M.
    Lindsay, Ross W. B.
    Davey, Dylan F.
    Flynn, Barbara J.
    Hoff, Soren T.
    Andersen, Peter
    Reed, Steven G.
    Morris, Sheldon L.
    Roederer, Mario
    Seder, Robert A.
    [J]. NATURE MEDICINE, 2007, 13 (07) : 843 - 850
  • [14] Recombinant NY-ESO-1 protein with ISCOMATRIX adjuvant induces broad integrated antibody and CD4+ and CD8+ T cell responses in humans
    Davis, ID
    Chen, WS
    Jackson, H
    Parente, P
    Shackleton, M
    Hopkins, W
    Chen, QY
    Dimopoulos, N
    Luke, T
    Murphy, R
    Scott, AM
    Maraskovsky, E
    McArthur, G
    MacGregor, D
    Sturrock, S
    Tai, TY
    Green, S
    Cuthbertson, A
    Maher, D
    Miloradovic, L
    Mitchell, SV
    Ritter, G
    Jungbluth, AA
    Chen, YT
    Gnjatic, S
    Hoffman, EW
    Old, LJ
    Cebon, JS
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (29) : 10697 - 10702
  • [15] ISCOMATRIX™ adjuvant for prophylactic and therapeutic vaccines
    Drane, Debbie
    Gittleson, Charmaine
    Boyle, Jeff
    Moraskovsky, Eugene
    [J]. EXPERT REVIEW OF VACCINES, 2007, 6 (05) : 761 - 772
  • [16] Drane Debbie, 2006, P191, DOI 10.1016/B978-012088403-2/50013-7
  • [17] Antibodies and CD4+ T-cells mediate cross-protection against H5N1 influenza virus infection in mice after vaccination with a low pathogenic H5N2 strain
    Droebner, Karoline
    Haasbach, Emanuel
    Fuchs, Cordula
    Weinzierl, Andreas O.
    Stevanovic, Stefan
    Buettner, Mathias
    Planz, Oliver
    [J]. VACCINE, 2008, 26 (52) : 6965 - 6974
  • [18] Polyfunctional T cell responses are a hallmark of HIV-2 infection
    Duvall, Melody G.
    Precopio, Melissa L.
    Ambrozak, David A.
    Jaye, Assan
    McMichael, Andrew J.
    Whittle, Hilton C.
    Roederer, Mario
    Rowland-Jones, Sarah L.
    Koup, Richard A.
    [J]. EUROPEAN JOURNAL OF IMMUNOLOGY, 2008, 38 (02) : 350 - 363
  • [19] Augmentation of human influenza A virus-specific cytotoxic T lymphocyte memory by influenza vaccine and adjuvanted carriers (ISCOMS)
    Ennis, FA
    Cruz, J
    Jameson, J
    Klein, M
    Burt, D
    Thipphawong, J
    [J]. VIROLOGY, 1999, 259 (02) : 256 - 261
  • [20] Multifunctional, high-level cytokine-producing Th1 cells in the lung, but not spleen, correlate with protection against Mycobacterium tuberculosis aerosol challenge in mice
    Forbes, Emily K.
    Sander, Clare
    Ronan, Edward O.
    McShane, Helen
    Hill, Adrian V. S.
    Beverley, Peter C. L.
    Tchilian, Elma Z.
    [J]. JOURNAL OF IMMUNOLOGY, 2008, 181 (07) : 4955 - 4964
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