Intramuscular Matrix-M-adjuvanted virosomal H5N1 vaccine induces high frequencies of multifunctional Th1 CD4+ cells and strong antibody responses in mice

被引:47
作者
Madhun, Abdullah S. [1 ]
Haaheim, Lars R. [1 ]
Nilsen, Mona V. [1 ]
Cox, Rebecca J. [1 ]
机构
[1] Univ Bergen, Gade Inst, Influenza Ctr, N-5021 Bergen, Norway
关键词
Multifunctional Th1; Matrix-M; Virosomal; Influenza; CYTOTOXIC T-LYMPHOCYTE; INFLUENZA-VIRUS INFECTION; CROSS-PROTECTION; MF59-ADJUVANTED INFLUENZA; ISCOMATRIX(TM) ADJUVANT; CANDIDATE VACCINES; IMMUNE-RESPONSES; RANDOMIZED-TRIAL; LETHAL CHALLENGE; ISCOM VACCINE;
D O I
10.1016/j.vaccine.2009.09.044
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Ideally, a candidate pandemic influenza vaccine should elicit rapid and strong cell-mediated and humoral immune responses, which are long-lasting and exhibit broad cross-reactivity against drifted strains. The present study investigated the detailed humoral and cellular immune responses in mice vaccinated intranasally or intramuscularly with inactivated influenza H5N1 (NIBRG-14) virosomal vaccine alone or formulated with Matrix-M adjuvant. The intramuscular Matrix-M-adjuvanted vaccine induced a strong immediate and long-term humoral immune response with high cross-reactivity against drifted H5N1 viruses and showed a close-sparing potential. Additionally, the vaccine induced a balanced Th1/Th2 cytokine profile and most importantly high frequencies of multifunctional Th1 CD4(+) cells. Our results highlight that Matrix-M adjuvant is a promising parenteral adjuvant for formulating pandemic candidate vaccines. (C) 2009 Elsevier Ltd. All rights reserved
引用
收藏
页码:7367 / 7376
页数:10
相关论文
共 60 条
  • [1] [Anonymous], 2009, WORLD NOW START 2009
  • [2] Cross-Protection against Lethal H5N1 Challenge in Ferrets with an Adjuvanted Pandemic Influenza Vaccine
    Baras, Benoit
    Stittelaar, Koert J.
    Simon, James H.
    Thoolen, Robert J. M. M.
    Mossman, Sally P.
    Pistoor, Frank H. M.
    van Amerongen, Geert
    Wettendorff, Martine A.
    Hanon, Emmanuel
    Osterhaus, Albert D. M. E.
    [J]. PLOS ONE, 2008, 3 (01):
  • [3] Effects of adjuvants on the safety and immunogenicity of an avian influenza H5N1 vaccine in adults
    Bernstein, David I.
    Edwards, Kathryn M.
    Dekker, Cornelia L.
    Belshe, Robert
    Talbot, Helen K. B.
    Graham, Irene L.
    Noah, Diana L.
    He, Fenhua
    Hill, Heather
    [J]. JOURNAL OF INFECTIOUS DISEASES, 2008, 197 (05) : 667 - 675
  • [4] The utility of ISCOMATRIX™ adjuvant for dose reduction of antigen for vaccines requiring antibody responses
    Boyle, Jeff
    Eastman, Donna
    Millar, Christine
    Camuglia, Sarina
    Cox, John
    Pearse, Martin
    Good, Jeremy
    Drane, Debbie
    [J]. VACCINE, 2007, 25 (14) : 2541 - 2544
  • [5] Induction of secretory immunity and memory at mucosal surfaces
    Brandtzaeg, Per
    [J]. VACCINE, 2007, 25 (30) : 5467 - 5484
  • [6] Safety and immunogenicity of an inactivated split-virion influenza A/Vietnam/1194/2004 (H5N1) vaccine:: phase I randomised trial
    Bresson, Jean-Louis
    Perronne, Christian
    Launay, Odile
    Gerdil, Catherine
    Saville, Melanie
    Wood, John
    Hoeschler, Katja
    Zambon, Maria C.
    [J]. LANCET, 2006, 367 (9523) : 1657 - 1664
  • [7] Virosome-mediated delivery of protein antigens in vivo: efficient induction of class I MHC-restricted cytotoxic T lymphocyte activity
    Bungener, L
    Huckriede, A
    de Mare, A
    de Vries-Idema, J
    Wilschut, J
    Daemen, T
    [J]. VACCINE, 2005, 23 (10) : 1232 - 1241
  • [8] Intranasal vaccination with ISCOMATRIX® adjuvanted influenza vaccine
    Coulter, A
    Harris, R
    Davis, R
    Drane, D
    Cox, J
    Ryan, D
    Sutton, P
    Rockman, S
    Pearse, M
    [J]. VACCINE, 2003, 21 (9-10) : 946 - 949
  • [9] Studies on experimental adjuvanted influenza vaccines:: comparison of immune stimulating complexes (Iscoms™) and oil-in-water vaccines
    Coulter, A
    Wong, TY
    Drane, D
    Bates, J
    Macfarlan, R
    Cox, J
    [J]. VACCINE, 1998, 16 (11-12) : 1243 - 1253
  • [10] A phase I clinical trial of a PER.C6® cell grown influenza H7 virus vaccine
    Cox, Rebecca J.
    Madhun, Abdullah S.
    Hauge, Solveig
    Sjursen, Haakon
    Major, Diane
    Kuhne, Mirjam
    Hoeschler, Katja
    Saville, Melanie
    Vogel, Frederick R.
    Barclay, Wendy
    Donatelli, Isabella
    Zambon, Maria
    Wood, John
    Haaheim, Lars R.
    [J]. VACCINE, 2009, 27 (13) : 1889 - 1897