Effect of fish meal supplementation on spatial distribution of lipid microdomains and on the lateral mobility of membrane-bound prostaglandin F2α receptors in bovine corpora lutea

被引：12

作者：

Plewes, M. R. ^{[1
]}

Burns, P. D. ^{[1
]}

Graham, P. E. ^{[1
]}

Bruemmer, J. E. ^{[2
]}

Engle, T. E. ^{[2
]}

Barisas, B. G. ^{[3
]}

机构：

[1] Univ Northern Colorado, Sch Biol Sci, Greeley, CO 80639 USA

[2] Colorado State Univ, Dept Anim Sci & Equine Sci, Ft Collins, CO 80523 USA

[3] Colorado State Univ, Dept Chem, Ft Collins, CO 80523 USA

来源：

DOMESTIC ANIMAL ENDOCRINOLOGY | 2017年 / 60卷

基金：

美国食品与农业研究所; 美国国家卫生研究院;

关键词：

Bovine; Corpus luteum; Omega-3 fatty acids; Lipid microdomains; FP receptor; LUTEINIZING-HORMONE RECEPTORS; SINGLE-PARTICLE TRACKING; POLYUNSATURATED FATTY-ACIDS; DIETARY ENERGY RESTRICTION; NONLACTATING BEEF-COWS; LACTATING DAIRY-COWS; CORPUS-LUTEUM; DOCOSAHEXAENOIC ACID; FOLLICULAR DEVELOPMENT; SIGNALING MOLECULES;

D O I：

10.1016/j.domaniend.2017.02.001

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

This study examined the effects of fish meal supplementation on spatial distribution of lipid microdomains and lateral mobility of prostaglandin F-2 alpha (FP) receptors on cell plasma membranes of the bovine corpus luteum (CL). Beef cows were stratified by BW and randomly assigned to receive a corn gluten meal supplement (n = 4) or fish meal supplement (n = 4) for 60 d to allow incorporation of fish meal-derived omega-3 fatty acids into luteal tissue. Ovaries bearing the CL were surgically removed between days 10 to 12 after estrus corresponding to approximately day 60 of supplementation. A 200-mg sample of luteal tissue was analyzed for fatty acid content using gas-liquid chromatography (GLC). The remaining tissue was enzymatically digested with collagenase to dissociate individual cells from the tissue. Cells were cultured to determine the effects of dietary supplementation on lipid microdomains and lateral mobility of FP receptors. Luteal tissue collected from fish meal-supplemented cows had increased omega-3 fatty acids content (P < 0.05). Lipid microdomain total fluorescent intensity was decreased in dissociated luteal cells from fish meal-supplemented cows (P < 0.05). Micro and macro diffusion coefficients of FP receptors were greater for cells obtained from fish meal-supplemented cows (P < 0.05). In addition, compartment diameter of domains was larger, whereas resident time was shorter for receptors from cells obtained from fish meal-supplemented cows (P < 0.05). Data indicate that dietary supplementation with fish meal increases omega-3 fatty acid content in luteal tissue causing disruption of lipid microdomains. This disruption leads to increased lateral mobility of the FP receptor, increased compartment sizes, and decreased resident time, which may influence prostaglandin signaling in the bovine CL (C) 2017 Elsevier Inc. All rights reserved.

引用

页码：9 / 18

页数：10

共 58 条

[11]

Burns P.D., 2002, Prof. Anim. Sci, V18, P373, DOI [10.15232/S1080-7446(15)31549-7, DOI 10.15232/S1080-7446(15)31549-7]

[12]

Burns PD, 1997, J ANIM SCI, V75, P1078

[13]

Burns PD, 2003, J ANIM SCI, V81, P2840

[14] Docosahexaenoic acid alters the size and distribution of cell surface microdomains [J].

Chapkin, Robert S. ;

Wang, Naisyin ;

Fan, Yang-Yi ;

Lupton, Joanne R. ;

Prior, Ian A. .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2008, 1778 (02) :466-471

[15] CELLULAR-DISTRIBUTION AND CYCLE PHASE DEPENDENCY OF GONADOTROPIN AND EICOSANOID BINDING-SITES IN BOVINE CORPORA-LUTEA [J].

CHEGINI, N ;

LEI, ZM ;

RAO, CV ;

HANSEL, W .

BIOLOGY OF REPRODUCTION, 1991, 45 (03) :506-513

[16] Effect of level of dietary n-3 polyunsaturated fatty acid supplementation on systemic and tissue fatty acid concentrations and on selected reproductive variables in cattle [J].

Childs, S. ;

Hennessy, A. A. ;

Sreenan, J. M. ;

Wathes, D. C. ;

Cheng, Z. ;

Stanton, C. ;

Diskin, M. G. ;

Kenny, D. A. .

THERIOGENOLOGY, 2008, 70 (04) :595-611

[17] Confined diffusion without fences of a G-protein-coupled receptor as revealed by single particle tracking [J].

Daumas, F ;

Destainville, N ;

Millot, C ;

Lopez, A ;

Dean, D ;

Salomé, L .

BIOPHYSICAL JOURNAL, 2003, 84 (01) :356-366

[18] Relationship of lipid rafts to transient confinement zones detected by single particle tracking [J].

Dietrich, C ;

Yang, B ;

Fujiwara, T ;

Kusumi, A ;

Jacobson, K .

BIOPHYSICAL JOURNAL, 2002, 82 (01) :274-284

[19] Embryonic and early foetal losses in cattle and other ruminants [J].

Diskin, M. G. ;

Morris, D. G. .

REPRODUCTION IN DOMESTIC ANIMALS, 2008, 43 :260-267

[20] Single-molecule microscopy reveals plasma membrane microdomains created by protein-protein networks that exclude or trap signaling molecules in T cells [J].

Douglass, AD ;

Vale, RD .

CELL, 2005, 121 (06) :937-950

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