Human hepatoma cell telomerase activity inhibition and cell cycle modulation by its RNA component antisense oligodeoxyribonucleotides

被引：0

作者：

Zhang, RG ^{[1
]}

Wang, XW ^{[1
]}

Yuan, JH ^{[1
]}

Xie, H ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Cell Biol, Shanghai 200031, Peoples R China

来源：

ACTA PHARMACOLOGICA SINICA | 2000年 / 21卷 / 08期

关键词：

telomerase; RNA; hepatocellular carcinoma; antisense oligodeoxyribonucleotides; cell cycle;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

AIM: To investigate the effects of human telomerase RNA component antisense oligodeoxyribonucleotides on telomerase activity of human hepatoma cells and their effects on cell cycle distribution. METHODS: Modified telomeric repeat amplification protocol was used to detect telomerase activity. Cell cycle was analyzed by flow cytometer. RESULTS: Telomerase activity was detected in all of four human hepatoma cell lines but absent in normal liver cells. Antisense oligomers to human telomerase RNA component (hTR) inhibited telomerase activity of BEL-7404 human hepatoma cells markedly in vitro. After in vitro treatment with antisense oligomers for 96 h, cell cycle of BEL-7404 human hepatoma cells was mainly arrested at G(2)/M phase. CONCLUSION: Anti- sense oligomers to hTR inhibited telomerase activity of BEL-7404 human hepatoma cells in vitro and resulted in cell cycle arrest at G(2)/M phase.

引用

页码：742 / 746

页数：5

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