Mapping of a QTL for Field Resistance to Blast (Pyricularia oryzae Cavara) in Ingngoppor-tinawon, a Rice (Oryza sativa L.) Landrace from the Philippines

被引：7
作者：
Mizobuchi, Ritsuko [1 ]
Sato, Hiroyuki [2 ]
Fukuoka, Shuichi [1 ]
Yamamoto, Shinichi [3 ]
Kawasaki-Tanaka, Akiko [4 ]
Fukuta, Yoshimichi [5 ]
机构：
[1] NIAS, Agrogen Res Ctr, Tsukuba, Ibaraki 3058602, Japan
[2] NARO, Kyushu Okinawa Agr Res Ctr KARC, Chikugo, Fukuoka 8330041, Japan
[3] NIAS, Genet Resources Ctr, Tsukuba, Ibaraki 3058602, Japan
[4] Tottori Univ, United Grad Sch Agr Sci, Tottori 6808553, Japan
[5] Japan Int Res Ctr Agr Sci, Ishigaki, Okinawa 9070002, Japan
来源：
JARQ-JAPAN AGRICULTURAL RESEARCH QUARTERLY | 2014年 / 48卷 / 04期
关键词：
rice breeding; SSR marker; quantitative trait loci; JAPANESE UPLAND RICE; NBS-LRR PROTEIN; DISEASE RESISTANCE; GENE; LINES; VARIETIES; PROMOTER; MARKERS; GENOME; KAHEI;
D O I：
10.6090/jarq.48.425
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
Rice blast, caused by Pyricularia oryzae Cavara (Magnaporthe grisea (Hebert) Barr), is one of the most severe global rice diseases. The Philippine rice landrace 'Ingngoppor-tinawon' (IT) displays not only high field resistance to rice blast but also high tolerance to lodging, despite its very long culms. These characteristics make IT suitable for use in breeding animal feed varieties as whole-crop silage (WCS). To characterize the blast resistance of IT, quantitative trait locus (QTL) analysis of the resistance using simple sequence repeat (SSR) markers was conducted in F-3 progenies derived from crossing IT with a susceptible lowland variety, 'Koshihikari'. A QTL for blast field resistance (qBFR4) was identified on chromosome 4, and the resistance allele was derived from IT. The QTL explained 73.5% of the phenotypic variation for blast resistance. qBFR4 mapped to almost the same position as that of the blast field-resistance gene Pi39. Inoculation assays revealed that IT (containing qBFR4) showed resistance characteristics that differed from those of Mineharuka (containing Pi39), making it very likely that resistance derived from IT is distinct from Pi39. IT showed broad resistance to Japanese isolates and race-specific resistance to some Philippine isolates.
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页码：425 / 431
页数：7
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