NLS-mediated NPC functions of the nucleoporin Pom121

被引：48
作者：
Yavuz, Sevil [1 ]
Santarella-Mellwig, Rachel [1 ]
Koch, Birgit [1 ]
Jaedicke, Andreas [1 ]
Mattaj, Iain W. [1 ]
Antonin, Wolfram [1 ]
机构：
[1] European Mol Biol Lab, D-69117 Heidelberg, Germany
来源：
FEBS LETTERS | 2010年 / 584卷 / 15期
关键词：
Pom121; Nucleoporin; Importin; NUCLEAR-PORE COMPLEX; RAN GTPASE CYCLE; IMPORTIN-BETA; ENVELOPE; CHROMATIN; PROTEIN; GRADIENT; NDC1; ELYS;
D O I：
10.1016/j.febslet.2010.07.008
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
RanGTP mediates nuclear import and mitotic spindle assembly by dissociating import receptors from nuclear localization signal (NLS) bearing proteins. We investigated the interplay between import receptors and the transmembrane nucleoporin Pom121. We found that Pom121 interacts with importin alpha/beta and a group of nucleoporins in an NLS-dependent manner. In vivo, replacement of Pom121 with an NLS mutant version resulted in defective nuclear transport, induction of aberrant cytoplasmic membrane stacks and decreased cell viability. We propose that the NLS sites of Pom121 affect its function in NPC assembly both by influencing nucleoporin interactions and pore membrane structure. Structured summary: MINT-7951230: pom121 (uniprotkb:Q5EWX9) physically interacts (MI:0914) with nup155 (uniprotkb:075694), Nup133 (uniprotkb:Q8WUM0) and Importin beta (uniprotkb:Q14974) by pull down (MI:0096) MINT-7951210: pom121 (uniprotkb:Q5EWX9) physically interacts (MI:0915) with Importin alpha (uniprotkb:P52170) and Importin beta (uniprotkb:P52297) by pull down (MI:0096) MINT-7951183: pom121 (uniprotkb:Q5EWX9) physically interacts (MI:0914) with nup155 (uniprotkb:Q7ZWL0), nup160 (uniprotkb:P83722), nup205 (uniprotkb:Q642R6), nup93 (uniprotkb:Q7ZX96), Importin beta (uniprotkb:P52297) and nup62 (uniprotkb:Q91349) by pull down (MI:0096) MINT-7951416: pom121 (uniprotkb:Q5EWX9) physically interacts (MI:0914) with nup155 (uniprotkb:Q7ZWL0), nup93 (uniprotkb:Q7ZX96) and Importin beta (uniprotkb:P52297) by pull down (MI:0096) MINT-7951276: pom121 (uniprotkb:Q5EWX9) physically interacts (MI:0914) with nup155 (uniprotkb:Q7ZWL0), nup205 (uniprotkb:Q642R6), nup93 (uniprotkb:Q7ZX96), Importin beta (uniprotkb:P52297) and nup62 (uniprotkb:Q91349) by pull down (MI:0096) MINT-7951306, MINT-7951362: pom121 (uniprotkb:Q5EWX9) physically interacts (MI:0914) with nup155 (uniprotkb:Q7ZWL0), nup160 (uniprotkb:P83722), nup93 (uniprotkb:Q7ZX96), Importin beta (uniprotkb:P52297) and nup62 (uniprotkb:Q91349) by pull down (MI:0096) (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：3292 / 3298
页数：7
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