Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe

被引：545

作者：

Kim, Dong-Uk ^{[1
]}

Hayles, Jacqueline ^{[2
]}

Kim, Dongsup ^{[3
]}

Wood, Valerie ^{[2
,4
]}

Park, Han-Oh ^{[5
]}

Won, Misun ^{[1
]}

Yoo, Hyang-Sook ^{[1
]}

Duhig, Trevor ^{[2
]}

Nam, Miyoung ^{[1
]}

Palmer, Georgia ^{[2
]}

Han, Sangjo ^{[3
]}

Jeffery, Linda ^{[2
]}

Baek, Seung-Tae ^{[1
]}

Lee, Hyemi ^{[1
]}

Shim, Young Sam ^{[1
]}

Lee, Minho ^{[3
]}

Kim, Lila ^{[1
]}

Heo, Kyung-Sun ^{[1
]}

Noh, Eun Joo ^{[1
]}

Lee, Ah-Reum ^{[1
]}

Jang, Young-Joo ^{[1
]}

Chung, Kyung-Sook ^{[1
]}

Choi, Shin-Jung ^{[1
]}

Park, Jo-Young ^{[1
]}

Park, Youngwoo ^{[1
]}

Kim, Hwan Mook ^{[6
]}

Park, Song-Kyu ^{[6
]}

Park, Hae-Joon ^{[5
]}

Kang, Eun-Jung ^{[5
]}

Kim, Hyong Bai ^{[7
]}

Kang, Hyun-Sam ^{[8
]}

Park, Hee-Moon ^{[9
]}

Kim, Kyunghoon ^{[10
]}

Song, Kiwon ^{[11
]}

Bin Song, Kyung ^{[12
]}

Nurse, Paul ^{[2
,13
]}

Hoe, Kwang-Lae ^{[1
,6
]}

机构：

[1] Korea Res Inst Biosci & Biotechnol, Integrat Om Res Ctr, Taejon, South Korea

[2] Cancer Res UK, London Res Inst, London, England

[3] Korea Adv Inst Sci & Technol, Dept Bio & Brain Engn, Taejon 305701, South Korea

[4] Wellcome Trust Sanger Inst, Cambridge, England

[5] Bioneer Corp, Taejon, South Korea

[6] Korea Res Inst Biosci & Biotechnol, Bioevaluat Ctr, Ochang, Chungcheongbuk, South Korea

[7] Korea Univ, Dept Bioinformat & Biotechnol, Jochiwon, Chungnam, South Korea

[8] Seoul Natl Univ, Sch Biol Sci, Seoul, South Korea

[9] Chungnam Natl Univ, Dept Microbiol, Taejon, South Korea

[10] Kangwon Natl Univ, Div Life Sci, Chunchon, Kangwon Do, South Korea

[11] Yonsei Univ, Dept Biochem, Seoul 120749, South Korea

[12] Chungnam Natl Univ, Dept Food & Nutr, Taejon, South Korea

[13] Rockefeller Univ, New York, NY 10021 USA

来源：

NATURE BIOTECHNOLOGY | 2010年 / 28卷 / 06期

基金：

英国惠康基金;

关键词：

PROTEIN LOCALIZATION; GLOBAL ANALYSIS; SACCHAROMYCES; SCREEN; CONSERVATION; MECHANISMS; MUTATIONS; EFFICIENT; NETWORKS; MODULES;

D O I：

10.1038/nbt.1628

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We report the construction and analysis of 4,836 heterozygous diploid deletion mutants covering 98.4% of the fission yeast genome providing a tool for studying eukaryotic biology. Comprehensive gene dispensability comparisons with budding yeast-the only other eukaryote for which a comprehensive knockout library exists-revealed that 83% of single-copy orthologs in the two yeasts had conserved dispensability. Gene dispensability differed for certain pathways between the two yeasts, including mitochondrial translation and cell cycle checkpoint control. We show that fission yeast has more essential genes than budding yeast and that essential genes are more likely than nonessential genes to be present in a single copy, to be broadly conserved and to contain introns. Growth fitness analyses determined sets of haploinsufficient and haploproficient genes for fission yeast, and comparisons with budding yeast identified specific ribosomal proteins and RNA polymerase subunits, which may act more generally to regulate eukaryotic cell growth.

引用

页码：617 / U111

页数：9

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