Hybrid magnetic scaffolds: The role of scaffolds charge on the cell proliferation and Ca2+ ions permeation

被引:23
作者
Castro, Pollyana S. [1 ]
Bertotti, Mauro [1 ]
Naves, Alliny F. [1 ]
Catalani, Luiz Henrique [1 ]
Cornejo, Daniel R. [2 ]
Bloisi, Georgia D. [1 ]
Petri, Denise F. S. [1 ]
机构
[1] Univ Sao Paulo, Inst Chem, Dept Fundamental Chem, Av Prof Lineu Prestes 748, BR-05508000 Sao Paulo, SP, Brazil
[2] Univ Sao Paulo, Inst Phys, Sao Paulo, SP, Brazil
基金
巴西圣保罗研究基金会;
关键词
Magnetic scaffolds; Xanthan; HPMC; Ca2+ ions permeation; Cell adhesion; Cell proliferation; NANOPARTICLES; BIOMATERIALS; XANTHAN; ENERGY; ORGANIZATION; PROMOTION; HYDROGEL; ACID;
D O I
10.1016/j.colsurfb.2017.05.046
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Magnetic scaffolds with different charge densities were prepared using magnetic nanoparticles (MNP) and xanthan gum (XG), a negatively charged polysaccharide, or hydroxypropyl methylcellulose (HPMC), an uncharged cellulose ether. XG chains were crosslinked with citric acid (cit), a triprotic acid, whereas HPMC chains were crosslinked either with cit or with oxalic acid (oxa), a diprotic acid. The scaffolds XG-cit, HPMC-cit and HPMC-oxa were characterized by scanning electron microscopy (SEM), inductively coupled plasma atomic emission spectroscopy (ICP-AES), superconducting quantum interference device (SQUID) magnetometry, contact angle and zeta-potential measurements. In addition, the flux of Ca2+ ions through the scaffolds was monitored by using a potentiometric microsensor. The adhesion and proliferation of murine fibroblasts (NIH/3T3) on XG-cit, XG-cit-MNP, HPMC-cit, HPMC-cit-MNP, HPMC-oxa and HPMC-oxa-MNP were evaluated by KIT assay. The magnetic scaffolds presented low coercivity (<25 Oe). The surface energy values determined for all scaffolds were similar, ranging from 43 mJ m(-2) to 46 mJ m(-2). However, the polar component decreased after MNP incorporation and the dispersive component of surface energy increased in average 1 mJ m(-2) after MNP incorporation. The permeation of Ca2+ ions through XG-cit-MNP was significantly higher in comparison with that on XG-cit and HPMC-cit scaffolds, but through HPMC-cit-MNP, HPMC-oxa and HPMC-oxa-MNP scaffolds it was negligible within the timescale of the experiment. The adhesion and proliferation of fibroblasts on the scaffolds followed the trend: XG-cit-MNP > XG-cit > HPMC-cit, HPMC-cit-MNP, HPMC-oxa, HPMC-oxa-MNP. A model was proposed to explain the cell behavior stimulated by the scaffold charge, MNP and Ca2+ ions permeation. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码:388 / 396
页数:9
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