Comparison of Storage Methods for Microfluidically Produced Water-in-Oil Droplets

被引：3

作者：

Groesche, Maximilian ^{[1
]}

Korvink, Jan G. ^{[2
]}

Rabe, Kersten S. ^{[1
]}

Niemeyer, Christof M. ^{[1
]}

机构：

[1] KIT, Inst Biol Interfaces IBG 1, Hermann von Helmholtz Pl 1, D-76344 Eggenstein Leopoldshafen, Germany

[2] KIT, IMT, Hermann von Helmholtz Pl 1, D-76344 Eggenstein Leopoldshafen, Germany

来源：

CHEMICAL ENGINEERING & TECHNOLOGY | 2019年 / 42卷 / 10期

关键词：

Microfluidics; Off-chip storage; Proteins; Vertical storage chamber; Water-in-oil droplets; CELLS; NANOPARTICLES; FUTURE; CHIP;

D O I：

10.1002/ceat.201900075

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

Microfluidically produced water-in-oil droplets are an important platform for biochemical research. To investigate the structural integrity of droplets during transfer and storage processes, different methods were compared. Storage as isolated droplets inside plastic tubing or a designed microfluidic chamber led to moderate decreases in droplet volume but only slight changes in monodispersity, whereas bulk storage in an Eppendorf cup led to the complete loss of monodispersity. It is further demonstrated that on-chip storage of the droplets in a fluidic microcavity array avoids coalescence and enables a reduction in volume with the concurrent increase in the concentration of entrapped proteins, which is relevant for applications in life science.

引用

页码：2028 / 2034

页数：7

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