Expression Changes of Transforming Growth Factor-Beta1 and Thrombospondin-1 in Cavernous Tissues of Diabetic Rats

被引：15

作者：

Zhang, Xiao-Ming ^{[1
]}

Shi, Pei-Hua ^{[2
]}

Cao, Sai-Hong ^{[2
]}

Yu, He-Jun ^{[2
]}

Azad, Junaid ^{[1
]}

Ling, Shu-Cai ^{[1
]}

机构：

[1] Zhejiang Univ, Sch Med, Dept Anat & Cell Biol, Hangzhou 310058, Zhejiang, Peoples R China

[2] Zhejiang Univ, Sir Run Run Shaw Hosp, Dept Orthoped, Hangzhou 310058, Zhejiang, Peoples R China

来源：

UROLOGIA INTERNATIONALIS | 2010年 / 84卷 / 02期

关键词：

Transforming growth factor-beta 1; Thrombospondin-1; Cavernous tissue; Diabetes; Erectile dysfunction; KINASE PHOSPHATASE-1 MKP-1; ERECTILE DYSFUNCTION; TGF-BETA; PATHOPHYSIOLOGY; MYOCARDIUM; ACTIVATION; FIBROSIS; PATHWAY; DISEASE; MOUSE;

D O I：

10.1159/000277602

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

Objective: To assess transforming growth factor-beta 1 (TGF-beta 1) and thrombospondin-1 (TSP-1) expression in the cavernous tissue of rats with streptozotocin (STZ)-induced diabetes. Materials and Methods: Twenty male Sprague-Dawley rats were randomly divided into 2 groups: diabetics and controls. We injected STZ intraperitoneally to induce diabetes, and studied the alterations in TGF-beta 1 and TSP-1 expression in the cavernous tissue of the 2 groups by immunohistochemistry and real-time quantitative reverse transcription polymerase chain reaction. HE staining was also applied to determine morphological changes. Weight, blood sugar and urine sugar were measured before and after model induction in both groups. Results: Expression of TGF-beta 1 and TSP-1 increased significantly in the cavernous tissue of the diabetic rats compared to the control group. Conclusions: TGF-beta 1 and TSP-1 expression changes in cavernous tissues may play an important role in diabetic erectile dysfunction. Copyright (c) 2010 S. Karger AG, Basel

引用

页码：221 / 225

页数：5

共 23 条

[1] Sexual dysfunction 3 - Sexual dysfunction in men and women with endocrine disorders [J].

Bhasin, Shalender ;

Enzlin, Paul ;

Coviello, Andrea ;

Basson, Rosemary .

LANCET, 2007, 369 (9561) :597-611

[2] Vascular response to intra-arterial injury in the thrombospondin-1 null mouse [J].

Budhani, Faisal ;

Leonard, Katherine A. ;

Bergdahl, Andreas ;

Gao, Jimin ;

Lawler, Jack ;

Davis, Elaine C. .

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 2007, 43 (02) :210-214

[3] Thrombospondin-1 is an endogenous activator of TGF-β in experimental diabetic nephropathy in vivo [J].

Daniel, Christoph ;

Schaub, Kathrin ;

Amann, Kerstin ;

Lawler, Jack ;

Hugo, Christian .

DIABETES, 2007, 56 (12) :2982-2989

[4] Pathophysiology of erectile dysfunction [J].

de Tejada, IS ;

Angulo, J ;

Cellek, S ;

González-Cadavid, N ;

Heaton, J ;

Pickard, R ;

Simonsen, U .

JOURNAL OF SEXUAL MEDICINE, 2005, 2 (01) :26-39

[5] Critical role of endogenous thrombospondin-1 in preventing expansion of healing myocardial infarcts [J].

Frangogiannis, NG ;

Ren, G ;

Dewald, O ;

Zymek, P ;

Haudek, S ;

Koerting, A ;

Winkelmann, K ;

Michael, LH ;

Lawler, J ;

Entman, ML .

CIRCULATION, 2005, 111 (22) :2935-2942

[6] Alterations in the transforming growth factor (TGF)-β pathway as a potential factor in the pathogenesis of Peyronie's disease [J].

Haag, Simone M. ;

Hauck, Ekkehard W. ;

Szardening-Kirchner, Carolin ;

Diemer, Thorsten ;

Cha, Eun-Sook ;

Weidner, Wolfgang ;

Eickelberg, Oliver .

EUROPEAN UROLOGY, 2007, 51 (01) :255-261

[7] Erectile dysfunction in the elderly man [J].

Hauri, D .

UROLOGIA INTERNATIONALIS, 2003, 70 (02) :89-99

[8] Vascular proliferation and transforming growth factor-β expression in pre- and early stage of diabetes mellitus in Otsuka Long-Evans Tokushima fatty rats [J].

Hosomi, N ;

Noma, T ;

Ohyama, H ;

Takahashi, T ;

Kohno, M .

ATHEROSCLEROSIS, 2002, 162 (01) :69-76

[9]

Hu WL, 2004, ASIAN J ANDROL, V6, P111

[10]

Huang Xing, 2004, Zhonghua Nan Ke Xue, V10, P867

← 1 2 3 →