Over-expression of a glutathione S-transferase gene, GsGST, from wild soybean (Glycine soja) enhances drought and salt tolerance in transgenic tobacco

被引：116

作者：

Ji, Wei ^{[1
]}

Zhu, Yanming ^{[1
]}

Li, Yong ^{[1
]}

Yang, Liang ^{[1
]}

Zhao, Xiaowen ^{[1
]}

Cai, Hua ^{[1
]}

Bai, Xi ^{[1
]}

机构：

[1] NE Agr Univ, Plant Bioengn Lab, Harbin 150030, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2010年 / 32卷 / 08期

关键词：

Glutathione S-transferase; Soybean; Stress tolerance; Transgenic tobacco; Wild soybean;

D O I：

10.1007/s10529-010-0269-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Glycine soja is a species of soybean that survives in adverse environments including high salt and drought conditions. We constructed a cDNA library from G. soja seedlings treated with NaCl and isolated a glutathione S-transferase gene (GsGST: GQ265911) from the library. The cDNA encoding GsGST contains an open reading frame of 660 bp and the predicted protein belongs to the tau class of GST family proteins. Tobacco plants over-expressing the GsGST gene showed sixfold higher GST activity than wild-type plants. Transgenic tobacco plants exhibited enhanced dehydration tolerance. T-2 transgenic tobacco plants showed higher tolerance at the seedling stage than wild-type plants to salt and mannitol as demonstrated by longer root length and less growth retardation.

引用

页码：1173 / 1179

页数：7

共 18 条

[1] Over-expression of a glutathione S-transferase gene, GsGST, from wild soybean (Glycine soja) enhances drought and salt tolerance in transgenic tobacco
Wei Ji
Yanming Zhu
Yong Li
Liang Yang
Xiaowen Zhao
Hua Cai
Xi Bai
Biotechnology Letters, 2010, 32 : 1173 - 1179
[2] Over-expressing GsGST14 from Glycine soja enhances alkaline tolerance of transgenic Medicago sativa
Wang, Z. -Y.
Song, F. -B.
Cai, H.
Zhu, Y. -M.
Bai, X.
Ji, W.
Li, Y.
Hua, Y.
BIOLOGIA PLANTARUM, 2012, 56 (03) : 516 - 520
[3] Over-expression of ζ glutathione S-transferase in transgenic rice enhances germination and growth at low temperature
Takesawa, T
Ito, M
Kanzaki, H
Kameya, N
Nakamura, I
MOLECULAR BREEDING, 2002, 9 (02) : 93 - 101
[4] Enhanced tolerance to herbicide of rice plants by over-expression of a glutathione S-transferase
Hu, Tingzhang
Qv, Xiaoxiao
Xiao, Guosheng
Huang, Xiaoyun
MOLECULAR BREEDING, 2009, 24 (04) : 409 - 418
[5] Expression of SbGSTU (tau class glutathione S-transferase) gene isolated from Salicornia brachiata in tobacco for salt tolerance
Jha, Bhavanath
Sharma, Anubha
Mishra, Avinash
MOLECULAR BIOLOGY REPORTS, 2011, 38 (07) : 4823 - 4832
[6] Overexpression of the glutathione S-transferase gene from Pyrus pyrifolia fruit improves tolerance to abiotic stress in transgenic tobacco plants
D. Liu
Y. Liu
J. Rao
G. Wang
H. Li
F. Ge
C. Chen
Molecular Biology, 2013, 47 : 515 - 523
[7] Overexpression of the glutathione S-transferase gene from Pyrus pyrifolia fruit improves tolerance to abiotic stress in transgenic tobacco plants
Liu, D.
Liu, Y.
Rao, J.
Wang, G.
Li, H.
Ge, F.
Chen, C.
MOLECULAR BIOLOGY, 2013, 47 (04) : 515 - 523
[8] Overexpression of an alfalfa glutathione S-transferase gene improved the saline-alkali tolerance of transgenic tobacco
Du, Binghao
Zhao, Weidi
An, Yimin
Li, Yakun
Zhang, Xue
Song, Lili
Guo, Changhong
BIOLOGY OPEN, 2019, 8 (09):
[9] GmGSTU23 Encoding a Tau Class Glutathione S-Transferase Protein Enhances the Salt Tolerance of Soybean (Glycine max L.)
Li, Xingang
Pang, Yuanting
Zhong, Yiwang
Cai, Zhandong
Ma, Qibin
Wen, Ke
Nian, Hai
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023, 24 (06)
[10] Drought tolerance through over-expression of the expansin gene TaEXPB23 in transgenic tobacco
Li, Feng
Xing, Shichao
Guo, Qifang
Zhao, Meirong
Zhang, Jin
Gao, Qiang
Wang, Guiping
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JOURNAL OF PLANT PHYSIOLOGY, 2011, 168 (09) : 960 - 966

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