African and Asian strains of Zika virus differ in their ability to infect and lyse primitive human placental trophoblast

被引：51

作者：

Sheridan, Megan A. ^{[1
,2
]}

Balaraman, Velmurugan ^{[3
]}

Schust, Danny J. ^{[4
]}

Ezashi, Toshihiko ^{[1
,5
]}

Roberts, R. Michael ^{[1
,2
,5
]}

Franz, Alexander W. E. ^{[3
]}

机构：

[1] Univ Missouri, Bond Life Sci Ctr, Columbia, MO 65211 USA

[2] Univ Missouri, Dept Biochem, Columbia, MO 65211 USA

[3] Univ Missouri, Dept Vet Pathobiol, Columbia, MO 65211 USA

[4] Univ Missouri, Dept Obstet Gynecol & Womens Hlth, Columbia, MO USA

[5] Univ Missouri, Div Anim Sci, Columbia, MO 65211 USA

来源：

PLOS ONE | 2018年 / 13卷 / 07期

关键词：

EMBRYONIC STEM-CELLS; RUBELLA;

D O I：

10.1371/journal.pone.0200086

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Zika virus (ZIKV) drew worldwide attention when a recent epidemic was linked to fetal microcephaly. Here we used human embryonic stem cell derived trophoblasts as a model for primitive placental trophoblast to test the hypothesis that there are differences in how the two genetically distinct ZIKV lineages, African (AF) and Asian (AS), target the human placenta. Upon infection with three AF (ib-H30656, SEN/1984/41525-DAK, and MR-766) and three AS (FSS13025, Mexl-44, and PANcdc259249) ZIKV strains, we observed that severe placental cell lysis was only induced after infection with AF strains, while viral replication rates remained similar between both lineages. Differences in cytopathic effects (CPE) were not observed in Vero cells, indicating that the AF strains were not inherently superior at cell lysis. Taken together, we propose that infection with AF strains of ZIKV early in pregnancy would likely result in pregnancy loss, rather than allow further fetal development with accompanying brain damage. Our results also suggest that the long term laboratory-adapted MR766 strain does not behave aberrantly in cell culture relative to other AF lineage strains.

引用

页数：18