Spray-dried anti-Campylobacter bacteriophage CP30A powder suitable for global distribution without cold chain infrastructure

被引:29
作者
Carrigy, Nicholas B. [1 ]
Liang, Lu [2 ]
Wang, Hui [1 ]
Kariuki, Samuel [3 ]
Nagel, Tobi E. [4 ]
Connerton, Ian F. [2 ]
Vehring, Reinhard [1 ]
机构
[1] Univ Alberta, Dept Mech Engn, 10-203 Donadeo Innovat Ctr Engn,9211 116th St NW, Edmonton, AB T6G 1H9, Canada
[2] Univ Nottingham, Sch Biosci, Loughborough, Leics, England
[3] Kenya Govt Med Res Ctr, Ctr Microbiol Res, Nairobi, Kenya
[4] Phages Global Hlth, Oakland, CA USA
基金
英国生物技术与生命科学研究理事会;
关键词
Campylobacter jejuni; Global health; Particle engineering; Phage CP20; Phage CP30A; Shipping stability; JEJUNI COLONIZATION; RESPIRABLE POWDERS; PHAGE THERAPY; EPIDEMIOLOGY; STABILITY; DELIVERY;
D O I
10.1016/j.ijpharm.2019.118601
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Campylobacter jejuni is a leading cause of foodborne illness globally. In this study, a spray drying and packaging process was developed to produce a thermally-stable dry powder containing bacteriophages that retains biological activity against C. jejuni after long distance shipping at ambient temperature. Spray drying using a twin-fluid atomizer resulted in significantly less (p < 0.05) titer reduction than spray drying using a vibrating mesh nebulizer. The use of centrifugation and dilution of filtered bacteriophage lysate in the formulation step resulted in a significantly greater (p < 0.05) proportion of bacteriophages remaining active relative to use of no centrifugation and dilution. The spray-dried bacteriophage powder generated using leucine and trehalose as excipients was flowable, non-cohesive, and exhibited a high manufacturing yield. The powder retained its titer with no significant differences (p > 0.05) in biological activity after storage in suitable packaging for at least 3 weeks at room temperature and after ambient temperature shipping a total distance of approximately 19,800 km, including with a 38 degrees C temperature excursion. The bacteriophage powder therefore appears suitable for global distribution without the need for cold chain infrastructure.
引用
收藏
页数:9
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