Comparative Study of GeneXpert MTB/RIF Assay and Multiplex PCR Assay for Direct Detection of Mycobacterium tuberculosis in Suspected Pulmonary Tuberculosis Patients

被引:17
作者
Sah, Anil Kumar [1 ,2 ]
Joshi, Bishnu [2 ]
Khadka, Dhruba Kumar [3 ]
Gupta, Birendra Prasad [4 ]
Adhikari, Anurag [5 ]
Singh, Subodh Kant [2 ]
Rai, Ganesh [1 ]
Vaidya, Geeta Shrestha [1 ]
Rajbhandari, Reema [2 ]
Pant, Basant [2 ]
Rai, Shiba Kumar [1 ]
机构
[1] Shi Gan Intl Coll Sci & Technol, Kathmandu, Nepal
[2] Annapurna Res Ctr, Kathmandu, Nepal
[3] Natl TB Ctr, Thimi, Bhaktapur, Nepal
[4] Tribhuvan Univ, Cent Dept Biotechnol, Kritipur, Nepal
[5] Kathmandu Res Inst Biol Sci, Mahalaxmi 19, Lalitpur, Nepal
关键词
POLYMERASE-CHAIN-REACTION; RAPID DIAGNOSIS; INSERTION-SEQUENCE; RIFAMPIN RESISTANCE; IS6110; IDENTIFICATION; CULTURE; DNA; PURIFICATION; MICROSCOPY;
D O I
10.1007/s00284-017-1279-x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Pulmonary tuberculosis (PTB) is one of the major infectious diseases in developing countries. The objective of this study was to compare rapid diagnostics technique, GeneXpert MTB/RIF (GeneXpert) and Multiplex PCR assay (MPCR) targeting IS6110 segment and mpb64 gene for direct detection of Mycobacterium tuberculosis (MTB) in suspected PTB patients. A cross sectional study was carried among 105 sputum samples from suspected PTB patients to evaluate GeneXpert and Multiplex PCR who visited National Tuberculosis Center, Nepal. The patient's sputum samples were used directly for the GeneXpert whereas DNA extraction by CTAB method was followed to process the sample for MPCR. The sensitivity and specificity of GeneXpert and MPCR in smear positive cases was 78.6, 33.3, and 100.0%, 66.7%, respectively (P = 0.125). However, in smear negative cases sensitivity and specificity of both methods exhibited 90.9, 95.2, and 100.0%, 100.0% respectively (P = 0.625). Finally, the sensitivity and specificity of GeneXpert and MPCR were 82.9, 95.3 and 100.0%, 98.5% respectively, (P = 0.549) in pulmonary cases. Comparatively, we observed higher sensitivity and specificity for MPCR than GeneXpert for both smear positive and negative samples. Thus, we recommend MPCR alongside GeneXpert for the better diagnostic accuracy of PTB in a resource-limited country where tuberculosis is endemic.
引用
收藏
页码:1026 / 1032
页数:7
相关论文
共 35 条
[11]   Performance evaluation of the Xpert MTB/RIF assay according to its clinical application [J].
Huh, Hee Jae ;
Jeong, Byeong-Ho ;
Jeon, Kyeongman ;
Koh, Won-Jung ;
Ki, Chang-Seok ;
Lee, Nam Yong .
BMC INFECTIOUS DISEASES, 2014, 14
[12]   Cepheid GeneXpert MTB/RIF Assay for Mycobacterium tuberculosis Detection and Rifampin Resistance Identification in Patients with Substantial Clinical Indications of Tuberculosis and Smear-Negative Microscopy Results [J].
Ioannidis, Panayotis ;
Papaventsis, Dimitrios ;
Karabela, Simona ;
Nikolaou, Stavroula ;
Panagi, Marina ;
Raftopoulou, Ekaterini ;
Konstantinidou, Eythymia ;
Marinou, Ioanna ;
Kanavaki, Sofia .
JOURNAL OF CLINICAL MICROBIOLOGY, 2011, 49 (08) :3068-3070
[13]   Rapid diagnosis of tuberculosis using Xpert MTB/RIF assay - Report from a developing country [J].
Iram, Shagufta ;
Zeenat, Asyia ;
Hussain, Shahida ;
Yusuf, Noshin Wasim ;
Aslam, Maleeha .
PAKISTAN JOURNAL OF MEDICAL SCIENCES, 2015, 31 (01) :105-110
[14]   Multiplex PCR assay for immediate identification of the infecting species in patients with mycobacterial disease [J].
Kox, LFF ;
Jansen, HM ;
Kuijper, S ;
Kolk, AHJ .
JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (06) :1492-1498
[15]   Diagnostic Accuracy and Turnaround Time of the Xpert MTB/RIF Assay in Routine Clinical Practice [J].
Kwak, Nakwon ;
Choi, Sun Mi ;
Lee, Jinwoo ;
Park, Young Sik ;
Lee, Chang-Hoon ;
Lee, Sang-Min ;
Yoo, Chul-Gyu ;
Kim, Young Whan ;
Han, Sung Koo ;
Yim, Jae-Joon .
PLOS ONE, 2013, 8 (10)
[16]   Advances in tuberculosis diagnostics: the Xpert MTB/RIF assay and future prospects for a point-of-care test [J].
Lawn, Stephen D. ;
Mwaba, Peter ;
Bates, Matthew ;
Piatek, Amy ;
Alexander, Heather ;
Marais, Ben J. ;
Cuevas, Luis E. ;
McHugh, Timothy D. ;
Zijenah, Lynn ;
Kapata, Nathan ;
Abubakar, Ibrahim ;
McNerney, Ruth ;
Hoelscher, Michael ;
Memish, Ziad A. ;
Migliori, Giovanni Battista ;
Kim, Peter ;
Maeurer, Markus ;
Schito, Marco ;
Zumla, Alimuddin .
LANCET INFECTIOUS DISEASES, 2013, 13 (04) :349-361
[17]   Sequence capture-PCR improves detection of mycobacterial DNA in clinical specimens [J].
Mangiapan, G ;
Vokurka, M ;
Shouls, L ;
Cadranel, J ;
Lecossier, D ;
vanEmbden, J ;
Hance, AJ .
JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (05) :1209-1215
[18]   Detection of 123 bp fragment of insertion element IS6110 Mycobacterium tuberculosis for diagnosis of extrapulmonary tuberculosis [J].
Maurya, A. K. ;
Kant, S. ;
Nag, V. L. ;
Kushwaha, R. A. S. ;
Dhole, T. N. .
INDIAN JOURNAL OF MEDICAL MICROBIOLOGY, 2012, 30 (02) :182-186
[19]   Comparative evaluation of IS6110 PCR via conventional methods in rapid diagnosis of new and previously treated cases of extrapulmonary tuberculosis [J].
Maurya, Anand Kumar ;
Kant, Surya ;
Nag, Vijaya Lakshmi ;
Kushwaha, Ram Awadh Singh ;
Kumar, Manoj ;
Dhole, Tapan N. .
TUBERKULOZ VE TORAK-TUBERCULOSIS AND THORAX, 2011, 59 (03) :213-220
[20]   The role of IS6110 in the evolution of Mycobacterium tuberculosis [J].
McEvoy, Christopher R. E. ;
Falmer, Alecia A. ;
Gey van Pittius, Nicolaas C. ;
Victor, Thomas C. ;
van Helden, Paul D. ;
Warren, Robin M. .
TUBERCULOSIS, 2007, 87 (05) :393-404