A novel low-cost and easy to develop functionalization platform. Case study: Aptamer-based detection of thrombin by surface plasmon resonance

被引：56

作者：

Polonschii, Cristina ^{[1
]}

David, Sorin ^{[1
]}

Tombelli, Sara ^{[2
]}

Mascini, Marco ^{[2
]}

Gheorghiu, Mihaela ^{[1
]}

机构：

[1] Int Ctr Biodynam, Bucharest 060101, Romania

[2] Univ Florence, Dept Chem, Sesto Fiorentino, Italy

来源：

TALANTA | 2010年 / 80卷 / 05期

关键词：

Carboxylated cross-linked BSA film; Thrombin; Aptamer; Surface plasmon resonance; Spreeta; MAGNETIC BEADS; IMMOBILIZATION; APTASENSOR; BINDING;

D O I：

10.1016/j.talanta.2009.11.023

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel low-cost platform to assess biomolecular interactions was investigated using surface plasmon resonance and an aptamer-based assay for thrombin detection. Gold SPR surface functionalized with a carboxylated cross-linked BSA film (cBSA) and commercially available carboxymethylated dextran chip (CM5) were used as immobilization platforms for the thrombin binding aptamer. The high end commercial instrument Biacore 3000 and a custom made FIA set-up involving TI Spreeta sensor (TSPR2K23) were used to assess different concentrations of thrombin within the range 01-150nM both in buffer and in a complex matrix (plasma) using the obtained aptasensors. Based on data derived from both CM5 and cBSA platforms, the cBSA aptasensor exhibited good selectivity, stability and regeneration ability, both in buffer and in complex matrices (plasma), comparable with CM5. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：2157 / 2164

页数：8

共 24 条

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[2]

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