Effects of dietary fat saturation on fatty acid composition and gene transcription in different tissues of Iberian pigs

被引：23

作者：

Benitez, R. ^{[1
]}

Nunez, Y. ^{[1
]}

Fernandez, A. ^{[1
]}

Isabel, B. ^{[2
]}

Fernandez, A. I. ^{[1
]}

Rodriguez, C. ^{[1
]}

Barragan, C. ^{[1
]}

Martin-Palomino, P. ^{[3
]}

Lopez-Bote, C. ^{[2
]}

Silio, L. ^{[1
]}

Ovilo, C. ^{[1
]}

机构：

[1] INIA, Dept Mejora Genet Anim, Madrid 28040, Spain

[2] UCM, Fac Vet, Dept Anim Prod, Madrid 28040, Spain

[3] Univ Alfonso X El Sabio, Madrid 28691, Spain

来源：

MEAT SCIENCE | 2015年 / 102卷

关键词：

Fatty acid; Dietary fat; Gene expression; Iberian pig; Nutrigenomics; ADIPOSE-TISSUE; MEAT QUALITY; CARCASS CHARACTERISTICS; LIPOGENIC GENES; FREE-RANGE; MUSCLE; LIVER; METABOLISM; EXPRESSION; ACCUMULATION;

D O I：

10.1016/j.meatsci.2014.12.005

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The effect of two diets, respectively enriched with SFA (S) and PUFA (P), on FA tissue composition and gene expression was studied in fattened Iberian pigs. The FA composition of adipose, muscular and liver tissues was affected by dietary treatment. S group showed higher MUFA and MUFA/SFA ratio and lower PUFA and n-6/n-3 ratio than P group in all analyzed tissues. In muscle and liver the extracted lipids were separated into neutral lipids and polar lipid fractions which showed significantly different responses to the dietary treatment, especially in liver where no significant effect of diet was observed in NL fraction. The expression of six candidate genes related to lipogenesis and FA oxidation was analyzed by qPCR. In liver, stearoyl CoA desaturase (SCD), acetyl CoA carboxylase alpha (ACACA) and malic enzyme 1 (ME1) genes showed higher expression in S group. SCD, ACACA,ME1, and fatty acid synthase (FASN) gene expression levels showed a wide variation across the tested tissues, with much higher expression levels observed in adipose tissue than other tissues. Tissue FA profile and gene expression results support the deposition of dietary FA, the lipogenic effect of dietary saturated fat in liver and the employment of saturated dietary fat for endogenous synthesis of MUFA in all the analyzed tissues. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：59 / 68

页数：10