The focal study was made on the determination trace elements contained in human hair of workers in paint factory, including chromium, iron, lead, and manganese. The hair sample was pretreated with organic solvent and by microwave digestion before their trace metals were measured with Graphite Furnace System Atomic absorption spectrophotometers. The result showed that total Ph, Mn and Iron levels in hair of workers in paint factory were significantly different from those in normal control subjects (P < 0.01), but the total. chromium level did not significant differ from that in normal control subjects(P > 0.05). The possible connection of these elements with the etiology of the worker is discussed.