Ginger essential oil and fractions against Mycobacterium spp.

被引：30

作者：

Baldin, Vanessa Pietrowski ^{[1
]}

de Lima Scodro, Regiane Bertin ^{[2
]}

Mariano Fernandez, Carla Maria ^{[3
]}

Ieque, Andressa Lorena ^{[2
]}

Caleffi-Ferracioli, Katiany Rizzieri ^{[1
]}

Dias Siqueira, Vera Lucia ^{[1
]}

de Almeida, Aryadne Larissa ^{[1
]}

Goncalves, Jose Eduardo ^{[4
]}

Garcia Cortez, Diogenes Aparicio ^{[3
]}

Cardoso, Rosilene Fressatti ^{[1
,2
]}

机构：

[1] Univ Estadual Maringa, Programa Posgrad Biociencias & Fisiopatol, Ave Colombo 5790, BR-87020900 Maringa, Parana, Brazil

[2] Univ Estadual Maringa, Programa Posgrad Ciencias Saude, Ave Colombo 5790, BR-87020900 Maringa, Parana, Brazil

[3] Univ Estadual Maringa, Programa Posgrad Ciencias Farmaceut, Ave Colombo 5790, BR-87020900 Maringa, Parana, Brazil

[4] Inst Cesumar Ciencias Tecnol & Inovacao ICETI, Av Guedner 1610, BR-87050390 Maringa, Parana, Brazil

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 2019年 / 244卷

关键词：

Mycobacteria; Essential oil; Ginger; Cytotoxicity; Terpenoids; ZINGIBER-OFFICINALE ROSCOE; NONTUBERCULOUS MYCOBACTERIA; ANTIMYCOBACTERIAL ACTIVITY; TUBERCULOSIS; SUSCEPTIBILITY; ANTIOXIDANT; TOXICITY; DRUGS; ASSAY;

D O I：

10.1016/j.jep.2019.112095

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Ethnopharmacological relevance: Zingiber officinale (ginger) is a perennial herbaceous plant native in tropical Asia and generally cultivated in most American tropical countries with widespread use in popular medicine. Ginger essential oil (GEO) has been reported to exhibit several biological activities, such as antimicrobial. Aims of the study: The aim of this study was to determine the composition and the property of GEO and related fractions against Mtb and NTM, as well as their cytotoxicity. Methods and materials: GEO was obtained by hydrodistillation and fractionation was performed. Chemical characterization of GEO and fractions were carried out by gas chromatography/mass spectrometry. The anti-mycobacterial activity was evaluated by resazurin microtiter assay plate and broth microdilution method for Mtb and NTM, respectively. The cytotoxicity in Vero cells was assessed by MTT colorimetric assay. Results: The analyses showed 63 compounds in the GEO sample, characterized by a high number of monoterpenes and sesquiterpenes. GEO fractionation rendered 11 fractions (FR1 to FR11). GEO and fractions minimum inhibitory concentration ranged from 31.25 to > 250 mu g/mL against Mtb and from 15.6 to > 250 mu g/mL against NTM. GEO showed better activity against NTM, M. chelonae, and M. abscessus sub. massiliense, than the semi-pure fractions. One fraction (FR5), containing gamma-eudesmol as the main compound, was the most active against Mtb and NTM. The GEO and semi-pure fractions cytotoxicity assay showed CC50 63.3 mu g/mL, and 36.3-312.5 mu g/mL, respectively. Conclusions: In general, GEO showed a mix of monoterpenes and sesquiterpenes and a better antimycobacterial activity than the semi-pure fractions. Cytotoxic effects of GEO and its fractions should be better investigated.

引用

页数：7

共 35 条

[1]

Adams R.P., 2012, IDENTIFICATION ESSEN

[2] Some phytochemical, pharmacological and toxicological properties of ginger (Zingiber officinale Roscoe): A review of recent research [J].

Ali, Badreldin H. ;

Blunden, Gerald ;

Tanira, Musbah O. ;

Nemmar, Abderrahim .

FOOD AND CHEMICAL TOXICOLOGY, 2008, 46 (02) :409-420

[3] Antimycobacterial Activity of Some Commercially Available Plant-Derived Essential Oils [J].

Alves, J. A. ;

Mantovani, A. L. L. ;

Martins, M. H. G. ;

Abrao, F. ;

Lucarini, R. ;

Crotti, A. E. M. ;

Martins, C. H. G. .

CHEMISTRY OF NATURAL COMPOUNDS, 2015, 51 (02) :353-355

[4] Anti-Mycobacterium tuberculosis activity of essential oil and 6,7-dehydroroyleanone isolated from leaves of Tetradenia riparia (Hochst.) Codd (Lamiaceae) [J].

Baldin, Vanessa Pietrowski ;

de Lima Scodro, Regiane Bertin ;

Lopes-Ortiz, Mariana Aparecida ;

de Almeida, Aryadne Larissa ;

Gazim, Zilda Cristiani ;

Ferarrese, Leticia ;

Faioes, Viviane dos Santos ;

Torres-Santos, Eduardo Caio ;

Agostinho Pires, Claudia Terencio ;

Caleffi-Ferracioli, Katiany Rizzieri ;

Dias Siqueira, Vera Lucia ;

Garcia Cortez, Diogenes Aparicio ;

Cardoso, Rosilene Fressatti .

PHYTOMEDICINE, 2018, 47 :34-39

[5] Essential Oils in Combination and Their Antimicrobial Properties [J].

Bassole, Imael Henri Nestor ;

Juliani, H. Rodolfo .

MOLECULES, 2012, 17 (04) :3989-4006

[6]

Bellik Y., 2014, Asian Pacific Journal of Tropical Disease, V4, P40

[7] Evaluation of Chemical Composition and Antileishmanial and Antituberculosis Activities of Essential Oils of Piper Species [J].

Bernuci, Karine Zanoli ;

Iwanaga, Camila Cristina ;

Mariano Fernandez-Andrade, Carla Maria ;

Lorenzetti, Fabiana Brusco ;

Torres-Santos, Eduardo Caio ;

Faioes, Viviane dos Santos ;

Goncalves, Jose Eduardo ;

do Amaral, Wanderlei ;

Deschamps, Cicero ;

de Lima Scodro, Regiane Bertin ;

Cardoso, Rosilene Fressatti ;

Baldin, Vanessa Pietrowski ;

Garcia Cortez, Diogenes Aparicio .

MOLECULES, 2016, 21 (12)

[8] Eudesmol Isomers Induce Caspase-Mediated Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells [J].

Bomfim, Diogo S. ;

Ferraz, Rosana P. C. ;

Carvalho, Nanashara C. ;

Soares, Milena B. P. ;

Pinheiro, Maria L. B. ;

Costa, Emmanoel V. ;

Bezerra, Daniel P. .

BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY, 2013, 113 (05) :300-306

[9]

Bueno J, 2011, NAT PROD COMMUN, V6, P1743

[10] Antimycobacterial plant terpenoids [J].

Cantrell, CL ;

Franzblau, SG ;

Fischer, NH .

PLANTA MEDICA, 2001, 67 (08) :685-694

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