ISOLATION AND CHARACTERIZATION OF 20 POLYMORPHIC MICROSATELLITE MARKERS FOR JUGLANS MANDSHURICA (JUGLANDACEAE)

被引：13

作者：

Chen, Chang-Mei ^{[1
]}

Han, Shi-Jie ^{[2
]}

Yuan, Shu-Sheng ^{[1
]}

Wang, Chun-Jing ^{[1
]}

Yu, Jing-Hua ^{[1
]}

机构：

[1] Northeast Forestry Univ, State Engn Lab Bioresource Ecoutilizat Heilongjia, Harbin 150040, Peoples R China

[2] Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Liaoning, Peoples R China

来源：

APPLICATIONS IN PLANT SCIENCES | 2013年 / 1卷 / 07期

关键词：

genetic conservation; Juglandaceae; Juglans mandshurica; microsatellite marker; simple sequence repeat; APOPTOSIS;

D O I：

10.3732/apps.1200009

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Premise of the study: Fifty microsatellite loci were developed for the endangered species Juglans mandshurica to investigate its genetic diversity and population structure. Methods and Results: In all, 50 microsatellite markers were isolated from J. mandshurica, using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol. Twenty of these polymorphic markers were assessed in samples collected from 98 individuals among five populations in northeastern China. Across all of the J. mandshurica samples, the number of alleles per locus ranged from one to 17. Conclusions: These new microsatellite loci will be useful for conservation genetics studies of J. mandshurica.

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