Post-transcriptional maturation of the S receptor kinase of Brassica correlates with co-expression of the S-Locus glycoprotein in the stigmas of two Brassica strains and in transgenic tobacco plants

被引:56
作者
Dixit, R [1 ]
Nasrallah, ME [1 ]
Nasrallah, JB [1 ]
机构
[1] Cornell Univ, Dept Plant Biol, Ithaca, NY 14853 USA
关键词
D O I
10.1104/pp.124.1.297
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The S-locus-encoded S receptor kinase (SRK) is an intrinsic plasma membrane protein that is viewed as the primary stigma determinant of specificity in the self-incompatibility response of Brassica spp. We analyzed two self-compatible mutant strains that express low levels of the S-locus glycoprotein (SLG), a cell wall-localized protein also encoded at the S locus that is coordinately expressed with SRK. We found that mutant stigmas synthesized wild-type levels of SRK transcripts but failed to produce SRK protein at any of the developmental stages analyzed. Furthermore, SRK was shown to form aberrant high-molecular mass aggregates when expressed alone in transgenic tobacco (Nicotiana tabacum) plants. This aggregation was prevented in tobacco plants that co-expressed SRK and SLG, but not in tobacco plants that co-expressed SRK and SLR1, an SLG-related secreted protein not encoded at the S locus. In analyses of protein extracts under reducing and non-reducing conditions, evidence of intermolecular association was obtained only for SLG, a fraction of which formed disulfide-linked oligomers and was membrane associated. The data indicate that, at least in plants carrying the S haplotypes we analyzed, SRK is an inherently unstable protein and that SLG facilitates its accumulation to physiologically relevant levels in Brassica stigmas.
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页码:297 / 311
页数:15
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