The effect of subcutaneous unfractionated heparin and low-molecular weight heparin toward modification of diabetic acute influence on surgical wound healing in rats

被引:1
作者
Shafagh, Shima [1 ]
Matini, Amirhasan [2 ]
Noureddini, Mahdi [3 ]
Sehat, Mojtaba [4 ]
Kalani, Alireza [1 ]
Gharavi, Majid Kalbasi [5 ]
机构
[1] Kashan Univ Med Sci, Sch Med, Dept Gen Surg, Esfahan, Iran
[2] Kashan Univ Med Sci, Sch Med, Dept Clin Pathol, Esfahan, Iran
[3] Kashan Univ Med Sci, Sch Med, Dept Physiol, Esfahan, Iran
[4] Kashan Univ Med Sci, Sch Med, Dept Epidemiol, Esfahan, Iran
[5] Isfahan Univ Med Sci, Dept Gen Surg, Esfahan, Iran
关键词
Diabetes; heparin; surgical wound healing; ANGIOGENESIS; REPAIR;
D O I
10.4103/atr.atr_9_20
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
摘要
Background and Objectives: Diabetes mellitus is one of the disturbing factors in surgical wound repair that recognizing these mechanisms, and modifying them can be useful in preventing surgical wound complications. The aim of this study is to assess the effect of unfractionated heparin (UH) and low-molecular weight heparin (LMWH) on diabetic wounds in the rats. Materials and Methods: This study as a clinical trial in the animal phase was done in two groups that each group consists of three subgroups containing eight rats in each. Initially, under anesthesia, the skin was incised surgically, and interventions with UH in the first subgroup of each group, LMWH in the second subgroup, and normal saline in the third subgroup were performed. In the first group on the 7th day and the second group on the 10th day, wound biopsy was taken and examined pathologically. Finally, data were analyzed using Kruskal-Wallis and one-way analysis of variance statically test. Results: The results of this study showed in every two groups, there was a statistically significant difference between the subgroups for fibroblast and vascularity status, but this difference was not significant for epithelialization and collagen level. In addition, wound length in both the groups had a significant difference between subgroups. Conclusion: In this study, there was a relative difference between the administration of UH and low-molecular weight and surgical wound healing in diabetic rats.
引用
收藏
页码:19 / 22
页数:4
相关论文
共 22 条
[1]   Decrease of collagen deposition in wound repair in type I diabetes independent of glycemic control [J].
Black, E ;
Vibe-Petersen, J ;
Jorgensen, LN ;
Madsen, SM ;
Ågren, MS ;
Holstein, PE ;
Perrild, H ;
Gottrup, F .
ARCHIVES OF SURGERY, 2003, 138 (01) :34-40
[2]   Effect of a low molecular weight heparin molecule, dalteparin, on cellular apoptosis and inflammatory process in an incisional wound-healing model [J].
Civelek, Ali ;
Ak, Koray ;
Kurtkaya, Ozlem ;
Tekeli, Atike ;
Isbir, Selim ;
Nargileci, Erol ;
Arsan, Sinan ;
Sav, Aydin .
SURGERY TODAY, 2007, 37 (05) :406-411
[3]   Wound healing - A literature review [J].
de Oliveira Gonzalez, Ana Cristina ;
Costa, Tila Fortuna ;
Andrade, Zilton de Araujo ;
Alves Peixoto Medrado, Alena Ribeiro .
ANAIS BRASILEIROS DE DERMATOLOGIA, 2016, 91 (05) :614-620
[4]   Comparison of effects of unfractionated heparin and low molecular weight heparin on skin wound healing of rats [J].
Durmaz, Can Engin ;
Ozkan, Aydin ;
Senel, Bugra ;
Uyar, Hasan Alper .
ACTA CIRURGICA BRASILEIRA, 2012, 27 (09) :639-644
[5]   Wound repair and regeneration: Mechanisms, signaling, and translation [J].
Eming, Sabine A. ;
Martin, Paul ;
Tomic-Canic, Marjana .
SCIENCE TRANSLATIONAL MEDICINE, 2014, 6 (265)
[6]   Heparin desulfation modulates VEGF release and angiogenesis in diabetic wounds [J].
Freudenberg, Uwe ;
Zieris, Andrea ;
Chwalek, Karolina ;
Tsurkan, Mikhail V. ;
Maitz, Manfred F. ;
Atallah, Passant ;
Levental, Kandice R. ;
Eming, Sabine A. ;
Werner, Carsten .
JOURNAL OF CONTROLLED RELEASE, 2015, 220 :79-88
[7]   Topical vascular endothelial growth factor accelerates diabetic wound healing through increased angiogenesis and by mobilizing and recruiting bone marrow-derived cells [J].
Galiano, RD ;
Tepper, OM ;
Pelo, CR ;
Bhatt, KA ;
Callaghan, M ;
Bastidas, N ;
Bunting, S ;
Steinmetz, HG ;
Gurtner, GC .
AMERICAN JOURNAL OF PATHOLOGY, 2004, 164 (06) :1935-1947
[8]  
Galvan L, 1996, J Wound Ostomy Continence Nurs, V23, P224, DOI 10.1016/S1071-5754(96)90095-9
[9]   Diminished neuropeptide levels contribute to the impaired cutaneous healing response associated with diabetes mellitus [J].
Gibran, NS ;
Jang, YC ;
Isik, FF ;
Greenhalgh, DG ;
Muffley, LA ;
Underwood, RA ;
Usui, ML ;
Larsen, J ;
Smith, DG ;
Bunnett, N ;
Ansel, JC ;
Olerud, JE .
JOURNAL OF SURGICAL RESEARCH, 2002, 108 (01) :122-128
[10]   Factors Affecting Wound Healing [J].
Guo, S. ;
DiPietro, L. A. .
JOURNAL OF DENTAL RESEARCH, 2010, 89 (03) :219-229