Structure and function of lipid-DNA complexes for gene delivery

被引:251
|
作者
Chesnoy, S [1 ]
Huang, L [1 ]
机构
[1] Univ Pittsburgh, Sch Med, Dept Pharmacol, Lab Drug Targeting, Pittsburgh, PA 15261 USA
来源
ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE | 2000年 / 29卷
关键词
plasmid DNA; cationic lipid; transfection; X-ray diffraction; toxicity;
D O I
10.1146/annurev.biophys.29.1.27
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Owing to the rapid development of in vivo applications for nonviral gene delivery vectors, it is necessary to have a better understanding of how the structure-activity relationships of these lipid-DNA complexes are affected by their environment. Indeed, research in gene therapy first focused on in vitro cell culture studies to determine the mechanisms involved in the delivery of DNA into the cell. New biophysical techniques such as electron microscopy and X-ray diffraction have been developed to discern the structure of the lipid-DNA complex. However, further studies have revealed discrepancies between optimal lipid-DMA formulations for in vitro transfection and for in vivo administration of these vectors. Furthermore, some immune stimulatory effects have been associated with in vivo lipid-DNA administration. This review summarizes the current state of knowledge on in vitro and in vivo lipid-DNA complex transfections. New prospects of vectors for in vivo gene transfer are also discussed.
引用
收藏
页码:27 / 47
页数:21
相关论文
共 50 条
  • [21] The potential role of proteoglycans in cationic lipid-mediated gene delivery - Studies of the interaction of cationic lipid-DNA complexes with model glycosaminoglycans
    Wiethoff, CM
    Smith, JG
    Koe, GS
    Middaugh, CR
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (35) : 32806 - 32813
  • [22] Quantification of free DNA in lipid-DNA complexes using FRET
    Madeira, C
    Loura, L
    Aires-Barros, R
    Fedorov, A
    Prieto, M
    BIOPHYSICAL JOURNAL, 2004, 86 (01) : 313A - 313A
  • [23] In vivo studies of gene expression via transient transgenesis using lipid-DNA delivery
    McClarrinon, M
    Gilkey, L
    Watral, V
    Fox, B
    Bullock, C
    Fradkin, L
    Liggitt, D
    Roche, L
    Bussey, LB
    Fox, E
    Gorman, C
    DNA AND CELL BIOLOGY, 1999, 18 (07) : 533 - 547
  • [24] Computational and analytical modeling of cationic lipid-DNA complexes
    Farago, Oded
    Gronbech-Jensen, Niels
    BIOPHYSICAL JOURNAL, 2007, 92 (09) : 3228 - 3240
  • [25] Osmotic pressure probes interactions in lipid-DNA complexes
    Harries, D
    Petrache, HI
    Danino, D
    Kesselman, E
    Parsegian, VA
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2005, 230 : U1171 - U1172
  • [26] pH-Sensitive Pegylated Cationic Lipid-DNA Complexes for Gene Delivery: Transfection Efficiency and Live Cell Imaging Studies
    Chan, Chia-Ling
    Majzoub, Ramsey
    Shirazi, Rahau S.
    Liang, Keng-San
    Ewert, Kai K.
    Safinya, Cyrus R.
    BIOPHYSICAL JOURNAL, 2012, 102 (03) : 501A - 502A
  • [27] Lipid-DNA and lipid-polyelectrolyte mesophases:: Structure and exchange kinetics
    Artzner, F
    Zantl, R
    Rädler, JO
    CELLULAR AND MOLECULAR BIOLOGY, 2000, 46 (05) : 967 - 978
  • [28] Transfection efficiency and toxicity following delivery of naked plasmid DNA and cationic lipid-DNA complexes to ovine lung segments
    Emerson, M
    Renwick, L
    Tate, S
    Rhind, S
    Milne, E
    Painter, H
    Boyd, AC
    McLachlan, G
    Griesenbach, U
    Cheng, SH
    Gill, DR
    Hyde, SC
    Baker, A
    Alton, EW
    Porteous, DJ
    Collie, DDS
    MOLECULAR THERAPY, 2003, 8 (04) : 646 - 653
  • [29] Mechanisms of protection of cationic lipid-DNA complexes during lyophilization
    Allison, SD
    Anchordoquy, TJ
    JOURNAL OF PHARMACEUTICAL SCIENCES, 2000, 89 (05) : 682 - 691