Amperometric immunosensor for prolactin hormone measurement using antibodies loaded on a nano-Au monolayer modified ionic liquid carbon paste electrode

被引：51

作者：

Beitollahi, Hadi ^{[1
]}

Nekooei, Samira ^{[2
]}

Torkzadeh-Mahani, Masoud ^{[3
]}

机构：

[1] Grad Univ Adv Technol, Dept Environm, Inst Sci & High Technol & Environm Sci, Kerman, Iran

[2] Grad Univ Adv Technol, Dept Chem, Kerman, Iran

[3] Grad Univ Adv Technol, Dept Biotechnol, Inst Sci & High Technol & Environm Sci, Kerman, Iran

来源：

TALANTA | 2018年 / 188卷

关键词：

Electrochemical immunoassay; Prolactin; Carbon paste electrode; Gold nanoparticles; Ionic liquid; O-Aminophenol; VOLTAMMETRIC DETERMINATION; GOLD NANOPARTICLES; ELECTROCHEMICAL DETERMINATION; SENSOR; IMMUNOASSAY; ACID; NOREPINEPHRINE; ACETAMINOPHEN; FLUORESCENCE; NANOTUBES;

D O I：

10.1016/j.talanta.2018.06.044

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The fabrication of a novel electrochemical immunosensor for rapid and precise determination of prolactin was carried out using carbon paste electrode (CPE) consist of ionic liquid (IL) and graphite. Gold nanoparticles were employed as a modifier on the surface of CPE to immobilize the prolactin antibody (anti-PRL). The immunoassay was set up by sandwiching the antigen between prolactin antibody and the polyclonal anti-human-prolactin antibody labeled with horseradish peroxidase (HRP-labeled anti-PRL) as secondary antibody, on the surface of modified CPE. The reaction between O-aminophenol (OAP) and H2O2 which is catalyzed by labeled HRP on the sandwich immunosensor generate a signal in differential pulse voltammetry (DPV) that is used to determine the concentration of prolactin. This immunosensor provides the measurement of prolactin concentration in a linear range of 25.0-2000.0 mIU L-1 with a detection limit 12.5 mIU L-1. Moreover, it is applicable in the clinical assay of prolactin due to its high sensitivity and acceptable stability.

引用

页码：701 / 707

页数：7

共 60 条

[1] Fabrication of a sensor for simultaneous determination of norepinephrine, acetaminophen and tryptophan using a modified carbon nanotube paste electrode [J].