A fluorescence spectroscopic study of the interaction between norfloxacin and DNA

The interaction between norfloxacin (NFA) and DNA was studied by fluorescence and UV spectra methods. The results of the experiment indicate that DNA can strongly decrease the fluorescence intensity of norfloxacin by static quenching. When the NFA is in the concentration range from 5.86 x 10(-7) to 1.95 x 10(-6) molL(-1), there is a linear relationship between the fluorescence intensity of the system of NFA + DNA and the concentration of DNA. Also, when the concentration of the NFA is 5.86 x 10(-6) molL(-1), there is good linearity in the range of DNA concentration from 2.0 X 10(-5) -2.22 x 10(-4) molL(-1). The effects of interference substances and optimization of procedure were investigated. Three synthetic samples were determined satisfactorily.