Characterization of a new Leishmania major strain for use in a controlled human infection model

被引：33

作者：

Ashwin, Helen ^{[1
]}

Sadlova, Jovana ^{[2
]}

Vojtkova, Barbora ^{[2
]}

Becvar, Tomas ^{[2
]}

Lypaczewski, Patrick ^{[3
]}

Schwartz, Eli ^{[4
]}

Greensted, Elizabeth ^{[1
]}

Van Bocxlaer, Katrien ^{[1
]}

Pasin, Marion ^{[5
]}

Lipinski, Kai S. ^{[5
]}

Parkash, Vivak ^{[1
]}

Matlashewski, Greg ^{[3
]}

Layton, Alison M. ^{[1
]}

Lacey, Charles J. ^{[1
]}

Jaffe, Charles L. ^{[6
]}

Volf, Petr ^{[2
]}

Kaye, Paul M. ^{[1
]}

机构：

[1] Univ York, Hull York Med Sch, York Biomed Res Inst, York, N Yorkshire, England

[2] Charles Univ Prague, Fac Sci, Dept Parasitol, Vinicna 7, Prague, Czech Republic

[3] McGill Univ, Dept Microbiol & Immunol, Montreal, PQ, Canada

[4] Tel Aviv Univ, Sackler Sch Med, Chaim Sheba Med Ctr, Ctr Geog Med & Trop Dis, Tel Aviv, Israel

[5] Vibalogics GmbH, Cuxhaven, Germany

[6] Hebrew Univ Jerusalem, Hadassah Med Sch, Jerusalem, Israel

来源：

NATURE COMMUNICATIONS | 2021年 / 12卷 / 01期

基金：

英国医学研究理事会;

关键词：

RNA VIRUS LRV; SAND FLIES; CUTANEOUS LEISHMANIASIS; VISCERAL LEISHMANIASIS; VACCINE; VECTOR; PARASITES; DONOVANI; IDENTIFICATION; TRANSMISSION;

D O I：

10.1038/s41467-020-20569-3

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Leishmaniasis is widely regarded as a vaccine-preventable disease, but the costs required to reach pivotal Phase 3 studies and uncertainty about which candidate vaccines should be progressed into human studies significantly limits progress in vaccine development for this neglected tropical disease. Controlled human infection models (CHIMs) provide a pathway for accelerating vaccine development and to more fully understand disease pathogenesis and correlates of protection. Here, we describe the isolation, characterization and GMP manufacture of a new clinical strain of Leishmania major. Two fresh strains of L. major from Israel were initially compared by genome sequencing, in vivo infectivity and drug sensitivity in mice, and development and transmission competence in sand flies, allowing one to be selected for GMP production. This study addresses a major roadblock in the development of vaccines for leishmaniasis, providing a key resource for CHIM studies of sand fly transmitted cutaneous leishmaniasis.

引用

页数：12

共 83 条

[31] Promastigote secretory gel from natural and unnatural sand fly vectors exacerbate Leishmania major and Leishmania tropica cutaneous leishmaniasis in mice [J].