We describe, in a system whose uniqueness is that the presence of pores allows the volume to vary as budding proceeds, how phase separation on the surface of spheres extrudes material in the process called "budding". The system is giant phospholipid vesicles (GUVs) containing phase-separated regions of DOPC (soft, liquid) and DPPC (stiff, gel), with cholesterol and without it. Budding is triggered by adding the cationic pore-forming peptide, melittin. Without cholesterol, fluorescence experiments show that melittin selectively binds to the liquid domains, inducing them to form mainly exocytotic monodisperse smaller vesicle buds of this same material, causing the parent GUV to shrink. The effect of cholesterol is to produce just a few large buds following domain coalescence, rather than numerous smaller monodisperse ones. Line tension is experimentally shown to be essential for budding in this multicomponent membrane.
机构:
Univ Illinois, Dept Chem, Urbana, IL 61801 USAUniv Illinois, Dept Chem, Urbana, IL 61801 USA
Anthony, Stephen M.
Granick, Steve
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Univ Illinois, Dept Chem, Urbana, IL 61801 USA
Univ Illinois, Dept Mat Sci & Engn, Urbana, IL 61801 USA
Univ Illinois, Dept Phys, Urbana, IL 61801 USAUniv Illinois, Dept Chem, Urbana, IL 61801 USA
机构:
Tel Aviv Univ, Sackler Fac Med, Dept Physiol & Pharmacol, IL-69978 Tel Aviv, Israel
Univ Barcelona, Fac Fis, Dept Estructura & Constituents Mat, E-08028 Barcelona, SpainTel Aviv Univ, Sackler Fac Med, Dept Physiol & Pharmacol, IL-69978 Tel Aviv, Israel
Campelo, Felix
McMahon, Harvey T.
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MRC, Mol Biol Lab, Cambridge CB2 2QH, EnglandTel Aviv Univ, Sackler Fac Med, Dept Physiol & Pharmacol, IL-69978 Tel Aviv, Israel
McMahon, Harvey T.
Kozlov, Michael M.
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Tel Aviv Univ, Sackler Fac Med, Dept Physiol & Pharmacol, IL-69978 Tel Aviv, IsraelTel Aviv Univ, Sackler Fac Med, Dept Physiol & Pharmacol, IL-69978 Tel Aviv, Israel
机构:
Univ Illinois, Dept Chem, Urbana, IL 61801 USAUniv Illinois, Dept Chem, Urbana, IL 61801 USA
Anthony, Stephen M.
Granick, Steve
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Dept Chem, Urbana, IL 61801 USA
Univ Illinois, Dept Mat Sci & Engn, Urbana, IL 61801 USA
Univ Illinois, Dept Phys, Urbana, IL 61801 USAUniv Illinois, Dept Chem, Urbana, IL 61801 USA
机构:
Tel Aviv Univ, Sackler Fac Med, Dept Physiol & Pharmacol, IL-69978 Tel Aviv, Israel
Univ Barcelona, Fac Fis, Dept Estructura & Constituents Mat, E-08028 Barcelona, SpainTel Aviv Univ, Sackler Fac Med, Dept Physiol & Pharmacol, IL-69978 Tel Aviv, Israel
Campelo, Felix
McMahon, Harvey T.
论文数: 0引用数: 0
h-index: 0
机构:
MRC, Mol Biol Lab, Cambridge CB2 2QH, EnglandTel Aviv Univ, Sackler Fac Med, Dept Physiol & Pharmacol, IL-69978 Tel Aviv, Israel
McMahon, Harvey T.
Kozlov, Michael M.
论文数: 0引用数: 0
h-index: 0
机构:
Tel Aviv Univ, Sackler Fac Med, Dept Physiol & Pharmacol, IL-69978 Tel Aviv, IsraelTel Aviv Univ, Sackler Fac Med, Dept Physiol & Pharmacol, IL-69978 Tel Aviv, Israel