Effect and mechanism of miR-34a on proliferation, apoptosis and invasion of laryngeal carcinoma cells

Objective: To discuss the effect and mechanism of miR-34a on the proliferation, apoptosis and invasion of laryngeal carcinoma cells. Methods: The laryngeal squamous carcinoma Hep2 cells were transiently transfected with miR-34a mimics and miR-34a NC. The MTT, colony-forming assay, Hoechst staining and AnnexinV-PI double staining flow cytometry were employed to detect the effect of miR-34a on the viability and apoptosis of laryngeal squamous carcinoma Hep2 cells; Transwell assay to defect the effect of miR-34a on the migration and invasion of laryngeal squamous carcinoma Hep2 cells; western blot and RTPCR assay to defect the effect of miR-34a mimics on the expression of survivin and Iii -67 mRNA in laryngeal squamous carcinoma Hep2 cells. Results: Compared with miR-34a NC group, the cell viability in miR-34 mimics group was significantly decreased (P<0.01), the cell apoptosis rate was significantly increased (P<0.01), the abilities of cell migration and invasion were significantly reduced (P<0.01) and the expression of survivin and Ki-67 mRNA was significantly decreased (P4.01). Conclusions: The increased expression of miR-34a can induce the apoptosis of Hep2 laryngeal carcinoma cells and inhibit the cell proliferation and invasion, which is related to the down -regulated expression of survivin and Ki-67.