Integrated microbiome-metabolomics analysis reveals the potential therapeutic mechanism of Zuo-Jin-Wan in ulcerative colitis

被引：18

作者：

Cai, Ying ^{[1
]}

Li, Siju ^{[1
]}

Zhang, Xiaojun ^{[2
]}

Cao, Xueqin ^{[1
]}

Liu, Deliang ^{[1
]}

Zhu, Yanglu ^{[1
]}

Ye, Simin ^{[1
]}

Xu, Zengmei ^{[1
]}

Liao, Qiongfeng ^{[3
,4
]}

Hong, Yanjun ^{[1
,5
]}

Xie, Zhiyong ^{[1
,5
]}

机构：

[1] Sun Yat sen Univ, Sch Pharmaceut Sci Shenzhen, Guangzhou 510006, Peoples R China

[2] Maternal & Child Hlth Hosp Yingde City, Dept Pharm, Qingyuan 513000, Peoples R China

[3] Guangzhou Univ Chinese Med, Sch Chinese Mat Med, Guangzhou 510006, Peoples R China

[4] Guangzhou Univ Chinese Med, Sch Chinese Mat Med, 232 Waihuan East Rd, Guangzhou, Peoples R China

[5] Sun Yat sen Univ, Sch Pharmaceut Sci Shenzhen, 132 Waihuan East Rd, Guangzhou, Peoples R China

来源：

PHYTOMEDICINE | 2022年 / 98卷

基金：

中国国家自然科学基金;

关键词：

Ulcerative colitis; Gut microbiota; Metabolomics; Zuo-Jin-Wan; INFLAMMATORY BOWEL DISEASES; GUT MICROBIOTA; INTESTINAL MICROBIOTA; FECAL MICROBIOTA; CROHNS-DISEASE; SUCCINATE; RESPONSES; HEALTH; SIGNAL;

D O I：

10.1016/j.phymed.2021.153914

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Background: Dysregulation in gut microbiota and host cometabolome contributes to the complicated pathology of ulcerative colitis (UC), while Zuo-Jin-Wan (ZJW), a traditional Chinese medicine has shown therapeutic effects against UC with its underlying mechanism remains elusive. Purpose: This study utilized an integrated analysis combining gut microbiome and host cometabolism to disclose the potential therapeutic mechanism of ZJW on dextran sulfate sodium (DSS)-induced UC in rats. Methods: We first evaluated the therapeutic effects of ZJW treatment in DSS-induced rat model. 16S rRNA sequencing, 1H NMR spectroscopy-based metabolomics and Spearman correlation analysis were conducted to explore the potential therapeutic mechanism during the treatment. Results: Our results showed that UC symptoms in ZJW rats were significantly attenuated. Marked decline in microbial diversity in ZJW group was accompanied by its correspondent function adjustment. Specific enrichment of genus Bacteroides, Sutterella, Akkermansia and Roseburia along with the major varying amino acid metabolism and lipid metabolism were observed meantime. Metabolic data further corroborated that ZJWrelated metabolic changes were basically gathered in amino acid metabolism, carbohydrate/energy metabolism and lipid metabolism. Of note, some biochemical parameters were deeply implicated with the discriminative microbial genera and metabolites involved in tricarboxylic acid (TCA) cycle and amino acid metabolism, indicating the microbiome-metabolome association in gut microbiota-metabolite-phenotype axis during UC treatment of ZJW. Conclusion: For the first time, integrated microbiome-metabolome analysis depicted that ZJW could alleviate DSS-induced UC in rats via a crosstalk between gut microbiota and host cometabolites.

引用

页数：10

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