Characterisation of Pseudomonas spp. isolated from foods

被引：92
作者：
Franzetti, Laura [1 ]
Scarpellini, Mauro [1 ]
机构：
[1] Univ Milan, Dipartimento Sci & Technol Alimentari & Microbiol, Sez Microbiol Agraria Alimentare Ecol, I-20133 Milan, Italy
来源：
ANNALS OF MICROBIOLOGY | 2007年 / 57卷 / 01期
关键词：
Pseudomonas fluorescens; enzymatic activity; ITS1;
D O I：
10.1007/BF03175048
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Putative Pseudomonas spp. (102 isolates) from different foods were first characterised by API 20NE and then tested for some enzymatic activities (lipase and lecithinase production, starch hydrolysis and proteolytic activity). However subsequent molecular tests did not always confirm the results obtained, thus highlighting the limits of API 20NE. Instead RFLP ITS1 and the sequencing of 16S rRNA gene grouped the isolates into 6 clusters: Pseudomonas fluorescens (cluster I), Pseudomonas fragi (cluster II and V) Pseudomonas migulae (cluster III), Pseudomonas aeruginosa (cluster IV) and Pseudomonas chicorii (cluster VI). The pectinolytic activity was typical of species isolated from vegetable products, especially Pseudomonas fluorescens. Instead Pseudomonas fragi, predominantly isolated from meat was characterised by proteolytic and lipolytic activities.
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页码：39 / 47
页数：9
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