The CaPti1-CaERF3 module positively regulates resistance of Capsicum annuum to bacterial wilt disease by coupling enhanced immunity and dehydration tolerance

被引：7

作者：

Shi, Lanping ^{[1
,2
,3
]}

Li, Xia ^{[1
,2
,3
]}

Weng, Yahong ^{[3
]}

Cai, Hanyang ^{[4
]}

Liu, Kaisheng ^{[1
,2
]}

Xie, Baixue ^{[1
,2
]}

Ansar, Hussain ^{[3
,5
]}

Guan, Deyi ^{[1
,2
,3
]}

He, Shuilin ^{[1
,2
,3
]}

Liu, Zhiqin ^{[1
,2
,3
]}

机构：

[1] Fujian Agr & Forestry Univ, Key Lab Appl Genet Univ Fujian Prov, Fuzhou 350002, Peoples R China

[2] Fujian Agr & Forestry Univ, Minist Educ Genet Breeding & Multiple Utilizat Cr, Key Lab, Fuzhou 350002, Peoples R China

[3] Fujian Agr & Forestry Univ, Coll Agr, Fuzhou 350002, Peoples R China

[4] Fujian Agr & Forestry Univ, Coll Life Sci, Fuzhou 350002, Peoples R China

[5] Ghazi Univ, Dept Plant Breeding & Genet, Dera Ghazi Khan 32200, Pakistan

来源：

PLANT JOURNAL | 2022年 / 111卷 / 01期

基金：

中国国家自然科学基金;

关键词：

pepper; Ralstonia solanacearum; kinase; transcription factor; trans-activation; ERF TRANSCRIPTION FACTOR; CELL-DEATH; OSMOTIC-STRESS; ABSCISIC-ACID; GCC BOX; DEFENSE RESPONSE; GENE-EXPRESSION; JASMONIC ACID; PLANT-DISEASE; E3; LIGASE;

D O I：

10.1111/tpj.15790

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Bacterial wilt, a severe disease involving vascular system blockade, is caused by Ralstonia solanacearum. Although both plant immunity and dehydration tolerance might contribute to disease resistance, whether and how they are related remains unclear. Herein, we showed that immunity against R. solanacearum and dehydration tolerance are coupled and regulated by the CaPti1-CaERF3 module. CaPti1 and CaERF3 are members of the serine/threonine protein kinase and ethylene-responsive factor families, respectively. Expression profiling revealed that CaPti1 and CaERF3 were upregulated by R. solanacearum inoculation, dehydration stress, and exogenously applied abscisic acid (ABA). They in turn phenocopied each other in promoting resistance of pepper (Capsicum annuum) to bacterial wilt not only by activating salicylic acid-dependent CaPR1, but also by activating dehydration tolerance-related CaOSM1 and CaOSR1 and inducing stomatal closure to reduce water loss in an ABA signaling-dependent manner. Our yeast two hybrid assay showed that CaERF3 interacted with CaPti1, which was confirmed using co-immunoprecipitation, bimolecular fluorescence complementation, and pull-down assays. Chromatin immunoprecipitation and electrophoretic mobility shift assays showed that upon R. solanacearum inoculation, CaPR1, CaOSM1, and CaOSR1 were directly targeted and positively regulated by CaERF3 and potentiated by CaPti1. Additionally, our data indicated that the CaPti1-CaERF3 complex might act downstream of ABA signaling, as exogenously applied ABA did not alter regulation of stomatal aperture by the CaPti1-CaERF3 module. Importantly, the CaPti1-CaERF3 module positively affected pepper growth and the response to dehydration stress. Collectively, the results suggested that immunity and dehydration tolerance are coupled and positively regulated by CaPti1-CaERF3 in pepper plants to enhance resistance against R. solanacearum.

引用

页码：250 / 268

页数：19