Degradation of Curcuminoids by in Vitro Pure Culture Fermentation

被引：48

作者：

Tan, Suryani ^{[1
,2
,3
]}

Rupasinghe, Thusitha W. T. ^{[3
,5
]}

Tull, Dedreia L. ^{[3
,5
]}

Boughton, Berin ^{[5
,6
]}

Oliver, Christine ^{[1
]}

McSweeny, Chris ^{[7
]}

Gras, Sally L. ^{[3
,4
]}

Augustin, Mary Ann ^{[1
]}

机构：

[1] CSIRO Food & Nutr Flagship, Werribee, Vic 3030, Australia

[2] Univ Melbourne, Dept Chem & Biomol Engn, Parkville, Vic 3010, Australia

[3] Univ Melbourne, Mol Sci & Biotechnol Inst Bio21, Parkville, Vic 3010, Australia

[4] Univ Melbourne, ARC Dairy Innovat Hub, Dept Chem & Biomol Engn, Parkville, Vic 3010, Australia

[5] Univ Melbourne, Metab Australia, Parkville, Vic 3010, Australia

[6] Univ Melbourne, Sch Bot, Parkville, Vic 3010, Australia

[7] CSIRO Agr Flagship, St Lucia, Qld 4067, Australia

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2014年 / 62卷 / 45期

关键词：

curcumin; curcuminoids; Escherichia coli; gut bacteria; metabolism; CHEMOPREVENTIVE AGENT CURCUMIN; METABOLISM; BIOAVAILABILITY; POLYPHENOLS;

D O I：

10.1021/jf5031168

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Colonic bacteria may mediate the transformation of curcuminoids, but studies of this metabolism are limited. Here, the metabolism of curcuminoids by Escherichia fergusonii (ATCC 35469) and two Escherichia coli strains (ATCC 8739 and DH10B) was examined in modified medium for colon bacteria (mMCB) with or without pig cecal fluid. LC-MS analysis showed that 16-37% of curcumin, 6-16% of demethoxycurcumin (DMC) and 7-15% of bis-demethoxycurcumin (Bis-DMC), and 715% of bis-demethoxycurcumin (Bis-DMC) were converted following 36 h of fermentation, with the amount of curcuminoids degraded varying depending on the bacterial strain and medium used. Three metabolites (dihydrocurcumin (DHC), tetrahydrocurcumin (THC), and ferulic acid (FA)) were found in fermentation cultures with all strains used. In addition, a compound with m/z [M H]- 470 was found and identified to be a curcumin adduct (curcuminl-cysteine), using accurate mass FT-ICR-MS. This study provides insights into the bacterial metabolism of curcuminoids.

引用

页码：11005 / 11015

页数：11

共 29 条

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